Summary
The ultrastructural appearance of organic plates lying in the alveoli of a freshwater species ofEuplotes is described and seen to be similar to those previously reported from the marine speciesE. vannus. Enzymatic digestion using pepsin and trypsin indicates that the plates in both species are mainly composed of protein with a fine coating of polysaccharides, as revealed using the Thiéry-technique for polysaccharide staining.
Similar content being viewed by others
References
Allen, R. D., 1978: Membranes of ciliates: ultrastructure, biochemistry, and fusion. In: Membrane fusion (Poste, A., Nicolson, A. L., eds.), pp. 657–763. New York: Elsevier/North Holland Biomedical Press.
Batisse, A., 1968: Les ultrastructures squelettiques chez certainThecacinetidae. Protistologica4, 477–492.
De Haller, G., Rouiller, C., 1961: La structure fine deChlorogonium elongatum. I. Étude systématique au microscope électronique. J. Protozool.8, 452–462.
Dodge, J. D., Crawford, R. M., 1970: A survey of thecal fine structure in the Dinophyceae. Bot. J. Linn. Soc.63, 53–67.
Fauré-Fremiet, E., André, J., 1968: L'organisation corticale des Ciliata. C. R. Acad. Sci. (Paris)266, 487–490.
— —, 1968: Calcification tégumentaire chez les ciliés du genreColeps. J. Microsc.7, 693–704.
Faust, M. A., 1974: Structure of the periplast ofCryptomonas ovata var. palustris. J. Phycol.10, 121–124.
Foissner, W., 1978:Euplotes moebiusi f.quadricirratus (Ciliophora, Hypotrichida). I. Die Feinstruktur des Cortex und der argyrophilen Strukturen. Arch. Protistenk.120, 86–117.
Franke, W. W., Krien, S., Brown, R. M., Jr., 1969: Simultaneous glutaraldehyde-osmium- tetroxid fixation with postosmication. An improved fixation procedure for electron microscopy of plant and animal cells. Histochemie19, 162–164.
Grain, J., Batisse, A., 1974: Étude ultrastructurale du cilié chonotricheChilodochona quennerstedti Wallengren, 1895. I. Cortex et structures buccales. J. Protozool.21, 95–111.
—,Iftode, Fryd-Versavel, G., 1979: Étude des infraciliatures somatique et buccale deBryophrya bavariensis et considérations systématiques. Protistologica15, 581–595.
Hausmann, K., Kaiser, J., 1979: Arrangement and structure of plates in the cortical alveoli of the hypotrich ciliate,Euplotes vannus. J. Ultrastruct. Res.67, 15–22.
Leedale, G. F., 1964: Pellicle structure inEuglena. Brit. phycol. Bull.2, 291–306.
Lueken, W., Sieger, M., 1966: Ein leicht zu züchtendes Wimpertier: Eine Meeresform aus der GattungEuplotes. Mikrokosmos55, 193–201.
Monneron, A., 1966: Utilisation de la pronase en cytochemie ultrastructurale. J. Microsc.5, 583–596.
—, 1966: Action de certaines enzymes sur tissus inclus en epon. J. Microsc.5, 697–714.
Nevo, Z., Sharon, N., 1969: The cell wall ofPeridinium westii, a non-cellulosic glucan. Biochim. biophys. Acta173, 161–175.
Sabatini, D. D., Bensch, K., Barnett, R. J., 1963: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol.17, 19–58.
Sołtynska, M. S., 1971: Morphology and fine structure ofChilodonella cucullulus (O.F.M.). Cortex and cytopharyngeal apparatus. Acta Protozool.9, 49–82.
Thiéry, J. P., 1967: Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microsc.6, 987–1018.
Tucker, J. G., 1971: Development and deployment of cilia, basal bodies, and other micro-tubular organelles in the cortex of the ciliateNassula. J. Cell Sci.9, 539–567.
Von Stosch, H. A., 1969: Dinoflagellaten aus der Nordsee. Helgol. wiss. Meeresunters.19, 558–577.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Böhm, P., Hausmann, K. Cytochemical investigations of the alveolar plates of theEuplotidae (Ciliophora, Hypotrichida) . Protoplasma 106, 309–316 (1981). https://doi.org/10.1007/BF01275561
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01275561