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A highly efficientin vitro regeneration methodology for mature Chinese tallow tree (Sapium sebiferum Roxb.)

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Abstract

A highly efficientin vitro regeneration methodology for mature chutese tallow tree (Sapium sebiferum Roxb.) has been developed. Shoot segments cultured on MS medium supplemented with 7.5 µM NAA produced light green callus. Optimum shoot differentiation resulted when callus was transferred to MS medium with 1 µM BA and 0.25 µM NAA. Shoot forming ability of callus was higher on MS medium compared to B5, half-MS or WPM. A continuous shoot harvest system at four-week intervals was established. Shoot yield continued for six months without loss of vigour. Regenerated shoots were rooted by culturing on half strength agar-gelled MS medium containing 1 µM IBA. Rooted plantlets were transferred to 1:1 soil vermiculite mixture and acclimatized with 67 % survival rate. Fully acclimatized plants were planted in the field, and performance is being evaluated.

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Abbreviations

2, 4- D:

2, 4-dichlorophenoxyacetic acid

NAA:

1 - napthaleneacetic acid

BA:

benzyladenine

Kn:

kinetin

2-ip:

6 - (γ, γ -dimethylallylamino)-purine

IBA:

indole-3-butyric acid

WPM:

woody plant medium (1980)

MS:

Murashige and Skoog (1962) medium

B5:

Gamborg et al's medium (1968)

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Authors and Affiliations

  1. G. B. Plant Institute of Himalayan Environment and Development, 263 643 U.P., Kosi, Almora, India

    E. A. Siril & U. Dhar

Authors
  1. E. A. Siril
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  2. U. Dhar
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Communicated by W. Parrott

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Siril, E.A., Dhar, U. A highly efficientin vitro regeneration methodology for mature Chinese tallow tree (Sapium sebiferum Roxb.). Plant Cell Reports 16, 83–87 (1996). https://doi.org/10.1007/BF01275456

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  • DOI: https://doi.org/10.1007/BF01275456

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