Summary
Ultraviolet light sensitive mutants ofE. coli defective at theuvrA,uvrB oruvrC locus showed increased sensitivity to the lethal effects of mitomycin C when compared with theuvr + parental strain. In addition, DNA breakdown after treatment of cells with either mitomycin C or with ultraviolet light was greater in the parental strain carrying the activeuvr + genes than inuvr mutants. Thus, injuries produced by either mitomycin C or by ultraviolet light may be repaired by the same molecular mechanism which has been proposed and which involves defect excision, single strand breakdown and reconstruction of the DNA.
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Boyce, R., andR. Setlow: A simple method of increasing the incorporation of thymidine into the deoxyribonucleic acid ofEscherichia coli. Biochim. biophys. Acta (Amst.)61, 618–620 (1962).
Boyce, R. P., andP. Howard-Flanders: Release of ultraviolet light induced thymine dimers from DNA inE. coli K-12. Proc. nat. Acad. Sci. (Wash.)51, 293–300 (1964).
Brookes, P., andP. D. Lawley: The reaction of mono and di-fuctional alkylating agents with nucleic acids. Biochem. J.80, 496–503 (1961).
Greenberg, J., andP. Woody-Karrer: Radiosensitivity inE. coli. J. gen. Microbiol.33, 283–292 (1963).
Harm, W.: On the relationship between host cell reactivation and UV-reactivation in UV-inactivated phages. Z. Vererbungsl.94, 67–79 (1963).
Iijima, T., andA. Hagawara: Mutagenic action of mitomycin C onE. coli. Nature (Lond.)185, 395–396 (1960).
Iyer, I. N., andW. Szybalski:A molecular mechanism of mitomycin action: linking of complementary DNA strands. Proc. nat. Acad. Sci. (Wash.)50, 355–362 (1963).
——, andW. Szybaski: Mitomycin and porfiromycin: chemical mechanism of activation and cross-linking of DNA. Science145, 55–58 (1964).
Lawyer, P. D., andP. Brookes: Further studies on the alkylation of nucleic acids and their constituent nucleotides. Biochem. J.89, 127–138 (1963).
Levine, M.: Effect of mitomyein C on interactions between temperate phages and bacteria. Virology13, 493–499 (1961).
Otsuji, N., andS. Okubo: Reactivation of ultraviolet- and nitrous acid inactivated phages by host cells. Virology12, 607–609 (1960).
Otsuji, W., M. Sekiguchi, T. Iijima, andT. Takagi: Introduction of phage formation in lysogenicE. coli K-12 by mitomycin C. Nature (Lond.)184, 1079–1080 (1959).
Reich, E., A. J. Shatkin, andE. L. Tatum: Bacteriocidal action of mitomycin C. Biochim. biophys. Acta (Amst.)53, 132–149 (1961).
Ross, W. C. J.: Biological alkylating agents, p. 83–84. London: Butterworths & Co. 1962.
Setlow, R. B., andW. L. Carrier: The disappearance of thymine dimers from DNA: an error-correcting mechanism. Proc. nat. Acad. Sci. (Wash.)51, 226–231 (1964).
Straus, B., andR. Wahl: The presence of breaks in the deoxyribonucleic acid ofBacillus subtilis treatedin vivo with the alkylating agent, methyl-methanesulfonate. Biochim. biophys. Acta (Amst.)80, 116–126 (1964).
Szybalski, W.: Special microbiloogical systems. II. Observations on chemical mutagenesis in microorganisms. Ann. N. Y. Acad. Sci.76, 475–489 (1958).
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Boyce, R.P., Howard-Flanders, P. Genetic control of DNA breakdown and repair inE. coli K-12 treated with mitomycin C or ultraviolet light. Zeitschrift für Vererbungslehre 95, 345–350 (1964). https://doi.org/10.1007/BF01268667
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DOI: https://doi.org/10.1007/BF01268667