Summary
A simplified, less expensive cryopreservation technique for parathyroid tissue was examined in animal study and on human tissue in vitro. More than 3/4 of all replantations in the rat were succesful after cryopreservation. After replantation of one single gland only 1/3 of all animals attained normal parathyroid function. This fact shows the importance of the quantity of transplanted viable tissue. Viability of human parathyroid tissue after cryopreservation was 80–90%. The PTH-production of human parathyroid tissue in vitro after cryopreservation and the suppressibility in different calcium concentrations were similar to normal fresh tissue. All our results prove a good functioning of this simplified cryopreservation method.
Zusammenfassung
Eine vereinfachte und billigere Methode der Kryopreservation von Nebenschilddrüsengewebe wird im Tierversuch und an menschlichem Gewebe geprüft. Gber 3/4 aller Replantationen waren nach Kryopreservation bei Ratten erfolgreich. Wurde jeweils nur eine Drüse transplantiert, sank die Erfolgsquote auf 1/3, was darauf hinweist, daß die Menge des transplantierten Gewebes eine wesentliche Rolle spielt. Das kryopreservierte menschliche Nebenschilddrüsengewebe wies eine Vitalitätsrate von 80–90% auf. Die PTH-Produktion von kryopreserviertem Gewebe in vitro, sowie die Suppressibilität in verschiedenen Calciumkonzentrationen entsprechen dem Verhalten von frischem Gewebe. Die hier geprüfte Methode der Gefrierkonservierung kann anhand der Resultate als tauglich angesehen werden.
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References
Basile C, Lacour B, Drüeke T, Chanard J (1980) Successful parathyroid gland autotransplantation in the rat using a simplified cryopreservation technique. Surgery 88:677–681
Basile C, Drüeke T, Lacour B, Ulmann A, Bourdeau A, Utzinger B, Dubost C (1984) Total parathyroidectomy and delayed parathyroid autotransplantation using a simplified cryopreservation technique: Human and animal studies. Am J Kidney Dis 3:366–370
Blake DP, O'Brien T, Smith C, Andersen R, Hitchcock C (1983) Surgical treatment of renal hyperparathyroidism. Surg Gynecol Obstet 157:325–331
Brennan M, Brown E, Sears H, Aurbach G (1978) Human parathyroid cryopreservation: In vitro testing of function by parathyroid hormone release. Ann Surg 187:366–370
Brennan M, Brown E (1980) Prediction of in vivo function of human parathyroid tissue autografts by in vitro testing. World J Surg 4:747–753
Brennan M, Brown E, Spiegel A, Marx S, Doppman J, Jones D, Aurbach G (1979) Autotransplantation of cryopreserved parathyroid tissue in man. Ann Surg 189:139–142
Brown E, Hurwitz S, Aurbach G (1976) Preparation of viable isolated bovine parathyroid cells. Endocrinology 99:1582–1588
Buchmann P, Keusch G, Ittner J, Binswanger U, Largiadèr F (1984) Tertiary hyperparathyroidism after cadaver-kidney transplantation. Transplant Proc 16:1324
Diethelm A, Adams P, Murad T, Daniel W, Whelchel J, Rutzky E, Rostand S (1981) Treatment of secondary hyperparathyroidism in patients with chronic renal failure by total parathyroidectomy and parathyroid autograft. Ann Surg 193:777–791
Leight G, Parker G, Sears H, Marx S, Terrill R (1978) Experimental cryopreservation and autotransplantation of parathyroid glands. Ann Surg 188:16–21
Mallette L, Eisenberg K, Schwaitzberg S, Noon G (1984) Factors that influence the assessment of parathyroid function. Ann Surg 199:192–196
Mozes M, Soper W, Jonasson O, Lang G (1980) Total parathyroidectomy and autotransplantation in secondary hyperparathyroidism. Arch Surg 115:378–384
Rothmund M, Wagner PK, Gunther R (1982) Reoperationen bei persistierendem und rezidivierendem Hyperparathyroidismus. Langenbecks Arch Chir 356:105
Saxe A, Brennan F (1982) Reoperative parathyroid surgery for primary hyperparathyroidism caused by multiple-gland disease: Total parathyroidectomy and autotransplantation with cryopreserved tissue. Surgery 91:616–621
Saxe A, Spiegel A, Marx S, Brennan M (1982) Deferred parathyroid autografts with cryopreserved tissue after reoperative parathyroid surgery. Arch Surg 117:538–543
Mozes M, Soper W, Jonasson O, Lang G (1980) Total parathyroidectomy and autotransplantation in secondary hyperparathyroidism. Arch Surg 115:378–384
Takagi H, Tominaga Y, Uchida K, Yamada N, Kawai M, Kano T, Morimoto T (1984) Subtotal versus total parathyroidectomy with forearm autograft for secondary hyperparathyroidism in chronic renal failure. Ann Surg 200:18–23
Wagner PK, Rothmund M (1985) Autotransplantation von kältekonserviertem menschlichem Nebenschilddrüsengewebe. Chirurg 56:30–35
Wells S, Christiansen C (1970) The transplanted parathyroid gland: Evaluation of cryopreservation and other environmental factors which affect its function. Surgery 75:49–55
Welsh C, Taylor G, Cattell W, Baker L (1984) Parathyroid surgery in chronic renal failure: subtotal parathyroidectomy or autotransplantation? Br J Surg 71:591–592
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Duff, C., Largiadèr, F. Kryopreservation von Nebenschilddrüsengewebe: Prüfung einer vereinfachten Methode. Langenbecks Arch Chiv 367, 227–234 (1986). https://doi.org/10.1007/BF01263403
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DOI: https://doi.org/10.1007/BF01263403