Summary
Changes in the glycogen content and patterns of deposition in the developing rat brain were studied using a light microscopic periodic acid-Schiff method on embryonic days (ED) 14, 16, 18, 20 and postnatal days (PD) 1, 3, 7, 12, 16 and 21. Regional and temporal differences were quantified with an automatic image analyser by estimation of stained material in subpial regions of cerebral cortex, thalamus, superior colliculus and medulla. The cellular localization of glycogen particles was investigated by electron microscopy on ED 18, ED 20 and PD 2. On ED 14 the first signs of glycogen storage were found in parts of the immature choroid plexus and in radial glial cells in the midbrain and medullary raphé. With advancing foetal age these structures retained their high capacity for glycogen storage but, in addition, an increasing number of radial fibres in most of the brain stem regions and in the cerebral cortex of older foetuses (ED 18–20) showed significant amounts of glycogen. The storage of glycogen in cerebral cortex was relatively low at all foetal age intervals studied. In new born animals the distribution of glycogen particles was similar to that found in the late foetal brain. A decrease of glycogen content commenced from PD 1 to 3 and reached the pattern of the adult brain between PD 7 and 21.
Glycogen storage by radial glial cells in the developing rat brain might indicate their possible role as an energy source in perinatal carbohydrate metabolism.
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References
Bachelard, H. S. (1969) Carbohydrates. InHandbook of Neurochemistry (edited byLajtha, A.), Vol. 1, pp. 25–31. New York: Plenum Press.
Bachelard, H. S. (1970) Control of carbohydrate metabolism. InHandbook of Neurochemistry (edited byLajtha, A.), Vol. 4, pp. 1–12. New York: Plenum Press.
Bignami, A. &Dahl, D. (1974) Astrocyte-specific protein and radial glia in the cerebral cortex of newborn rat.Nature 252, 55–6.
Chesler, A. &Himwich, H. E. (1943) The glycogen content of various parts of the central nervous system of dogs and cats at different ages.Archives of Biochemistry and Biophysics 2, 175–81.
Choi, B. H. &Lapham, L. W. (1978) Radial glia in the human fetal cerebrum: A combined Golgi, immunofluorescent and electron microscopic study.Brain Research 148, 295–311.
Coxon, R. V. (1970) Glycogen metabolism. InHandbook of Neurochemistry. (edited byLajtha, A.), Vol. 3, pp. 37–52. New York: Plenum Press.
Duffy, T. E., Kohle, S. J. &Vannucci, R. C. (1975) Carbohydrate and energy metabolism in perinatal rat brain: relation to survival and anoxia.Journal of Neurochemistry 24, 271–6.
Edwards, C. &Rogers, K. (1972) Some factors influencing brain glycogen in the neonate chick.Journal of Neurochemistry 19, 2759–66.
Freyer, K., Hartmann, G., Baumbach, H., Ermisch, A. &Hess, J. (1976) Auswertung von Autoradiogrammen und von Bildern biologischer Objekte mit dem elektronischen Bildauswertegerät “Densitron II”. Zu Arbeitsweise, Leistungsfähigkeit und Einsatzmöglichkeiten des Gerätes.Acta histochemica 55, 208–15.
Hess, J., Korn, U., Freyer, K. &Ermisch, A. (1976) Auswertung von Autoradiogrammen und von Bildern biologischer Objekte mit dem elektronischen Bildauswertegerät “Densitron II”. 2. Bestimmung der spezifischen und Gesamtradioaktivität einzelner Zellen.Acta histochemica 56, 180–7.
Kohle, S. J. &Vannucci, R. C. (1977) Glycogen metabolism in fetal and postnatal rat brain: influence of birth.Journal of Neurochemistry 28, 441–3.
Margolis, R. K., Preti, C., Lai, D. &Margolis, R. U. (1976) Developmental changes in brain glycoproteins.Brain Research 112, 363–9.
Oksche, A. (1958) Histologische Untersuchungen über die Bedeutung des Ependyms, der Glia und der Plexus chorioidei für den Kohlenhydratstoffwechsel des ZNS.Zeitschrift für Zettforschung und mikroskopische Anatomie 48, 74–129.
Oksche, A. (1961) Der histochemisch nachweisbare Glykogenaufbau und -abbau in den Astrozyten und Ependymzellen als Beispiel einer funktionsabbhängigen Stoffwechselaktivität der Neuroglia.Zeitschrift für Zellforschung und mikroskopische Anatomie 54, 307–61.
Peters, A. &Feldman, M. (1973) The cortical plate and molecular layer of the late rat fetus.Zeischrift für Anatomie und Entwicklungsgeschichte 141, 3–37.
Rakic, P. (1971) Guidance of neurons migrating to the fetal monkey neocortex.Brain Research 33, 471–6.
Rakic, P. (1972) Mode of cell migration to the superficial layers of fetal monkey neocortex.Journal of Comparative Neurology 145, 61–84.
Rinaudo, M. T. &Ponzetto, C. (1974) UDP-glucose-glycogen glucosyltransferase, phosphorylase, phosphoglucomutase and UDP-glucose phosphorylase in some chick tissues in the first eight days after hatching.International Journal of Biochemistry 5, 613–616.
Schmechel, D. E. &Rakic, P. (1979) A Golgi study of radial glial cells in developing monkey telencephalon: Morphogenesis and transformation into astrocytes.Anatomy and Embryology 156, 115–52.
Seress, L. (1980) Development and structure of the radial glia in the postnatal rat brain.Anatomy and Embryology 160, 213–26.
Sidman, R. L. &Rakic, P. (1973) Neuronal migration with special reference to developing human brain: a review.Brain Research 62, 1–35.
Strang, R. H. C. &Bachelard, H. S. (1971) Extraction purification and turnover of rat brain glycogen.Journal of Neurochemistry 18, 1067–76.
Wohlfarth-Bottermann, K.-E. (1957) Die Kontrastierung tierischer Zellen und Gewebe im Rahmen ihrer elektronenmikroskopischen Untersuchung an ultradünnen Schnitten.Naturwissenschaften 44, 287–8.
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Brückner, G., Biesold, D. Histochemistry of glycogen deposition in perinatal rat brain: importance of radial glial cells. J Neurocytol 10, 749–757 (1981). https://doi.org/10.1007/BF01262651
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DOI: https://doi.org/10.1007/BF01262651