Summary
Incorporation of precursors of RNA into the enucleated cells ofAcetabularia mediterranea andPolyphysa cliftonii has been studied under conditions which exclude the possibility of errors due to contamination of the preparations by nuclei, nuclear debris or microorganisms.
Radioactive RNA has been isolated from the chloroplast, mitochondrial and supernatant cytoplasm fractions of nucleated and enucleated cells. Sucrose density gradient centrifugation of the isolated labelled RNA produces a sedimentation profile of radioactivity which is similar to that of RNA isolated fromE. coli ribosomes.
Anion exchange chromatography of alkaline hydrolysates of the 23 s and 16 s ribosomal RNA fractions shows incorporation of labelled uracil into RNA in the form of 2′(3′)-UMP and 2′(3′)-CMP. Labelled guanosine is incorporated only as 2′(3′)-GMP.
A slowly sedimenting radioactivity peak has been chromatographed on a Hershey column and found to correspond to cold t-RNA.
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Schweiger, H.G., Dillard, W.L., Gibor, A. et al. RNA- synthesis inAcetabularia . Protoplasma 64, 1–12 (1967). https://doi.org/10.1007/BF01257378
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DOI: https://doi.org/10.1007/BF01257378