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RNA synthesis inAcetabularia

III. The kinetics of RNA synthesis in nucleate and enucleated cells

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Summary

  1. 1.

    The kinetics of RNA synthesis in nucleate and enucleated cells ofAcetabularia mediterranea have been compared.

  2. 2.

    Nucleate cells and cells 24 hours after enucleation show no differences in their kinetics nor in the sedimentation behavior of the newly-synthesized RNA isolated after various short intervals (≦120 min) of incubation in the presence of tritiated ribonucleosides which can be attributed to enucleation. This fact is explained by predominant extranuclear RNA synthesis.

  3. 3.

    The kinetics of the synthesis of the 23 s and 16 s ribosomal RNA peaks suggest that each of these peaks may be composed of two components. The possible origin of these components is discussed.

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References

  • Baltus, E., andJ. Quertier, 1966: A method for the extraction and characterization of RNA from subcellular fractions ofAcetabularia. Biochim. Biophys. Acta119, 192–194.

    PubMed  Google Scholar 

  • ,J. E. Edström, M. Janowski, J. Hanocq-Quertier, R. Tencer, andJ. Brachet, 1968: Base composition and metabolism of various RNA fractions inAcetabularia mediterranea. Proc. nat. Acad. Sci. (U.S.A.)59, 406–413.

    Google Scholar 

  • Berger, S., 1967a: RNA-Synthesis inAcetabularia. II. RNA-Synthesis in isolated chloroplasts. Protoplasma64, 13–25.

    PubMed  Google Scholar 

  • - 1967 b: RNA-Synthese in isolierten Chloroplasten vonAcetabularia. Doctoral thesis, University of Cologne.

  • Boardman, N. K., R. I. B. Francki, andS. G. Wildman, 1965: Protein synthesis by cellfree extracts from tobacco leaves. II. Association of activity with chloroplast ribosomes. Biochemistry4, 872–876.

    Google Scholar 

  • Brachet, J., H. Chantrenne etF. Vanderhaeghe, 1955: Recherches sur les interactions biochimiques entre le noyau et le cytoplasme chez les organismes unicellulaires. II.Acetabularia mediterranea. Biochim. Biophys. Acta18, 544–563.

    PubMed  Google Scholar 

  • Bramwell, M., andH. Harris, 1967: The origin of the polydispersity in sedimentation patterns of rapidly labelled nuclear ribonucleic acid. Biochem. J.103, 816–830.

    PubMed  Google Scholar 

  • Bray, G. A., 1960: A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analyt. Biochem.1, 279–285.

    Google Scholar 

  • Dillard, W. L., andH. G. Schweiger, 1968: Kinetics of RNA synthesis inAcetabularia. Biochim. Biophys. Acta169, 561–563.

    PubMed  Google Scholar 

  • Fraenkel-Conrat, H., B. Singer, andA. Tsugita, 1961: Purification of viral RNA by means of bentonite. Virology14, 54–58.

    PubMed  Google Scholar 

  • Gibor, A., andM. Izawa, 1963: The DNA content of the chloroplasts ofAcetabularia. Proc. nat. Acad. Sci. (U.S.A.)50, 1164–1169.

    Google Scholar 

  • Hämmerling, J., 1963: Nucleo-cytoplasmic interactions inAcetabularia and other cells. Ann. Rev. Plant Physiol.14, 65–92.

    Google Scholar 

  • Janowski, M., 1965: Synthèse chloroplastique d'acides nucléiques chezAcetabularia mediterranea. Biochim. Biophys. Acta103, 399–408.

    PubMed  Google Scholar 

  • , 1966: Detection of ribosomes and polysomes inAcetabularia mediterranea. Life Sciences5, 2113–2116.

    Google Scholar 

  • Kirby, K. S., 1962: Ribonucleic acids. II. Improved preparation of rat-liver ribonucleic acid. Biochim. Biophys. Acta55, 545–546.

    PubMed  Google Scholar 

  • Kissane, J. M., andE. Robbins, 1958: The fluorometric measurement of deoxyribonucleic acids in animal tissues with special reference to the central nervous system. J. Biol. Chem.236, 184–188.

    Google Scholar 

  • Loening, U. E., andJ. Ingle, 1967: Diversity of RNA components in green plant tissues. Nature215, 363–367.

    PubMed  Google Scholar 

  • Martin, R. G., andB. N. Ames, 1961: A method for determining the sedimentation behavior of enzymes: Application to protein mixtures. J. Biol. Chem.236, 1372–1379.

    PubMed  Google Scholar 

  • Naora, H., H. Naora, andJ. Brachet, 1960: Studies on independent synthesis of cytoplasmic ribonucleic acids inAcetabularia mediterranea, J. Gen. Physiol.43, 1083–1102.

    PubMed  Google Scholar 

  • Penman, S., I. Smith, andE. Holtzman, 1966: Ribosomal RNA synthesis and processing in a particulate site in the HeLa cell nucleus. Science154, 786–789.

    PubMed  Google Scholar 

  • Ralph, R. K., andA. R. Bellamy, 1964: Isolation and purification of undegraded ribonucleic acids. Biochim. Biophys. Acta87, 9–16.

    PubMed  Google Scholar 

  • Rodriguez-Lopez, M., andD. Vazquez, 1968: Comparative studies on cytoplasmic ribosomes from algae. Life Sciences7, 327–336.

    PubMed  Google Scholar 

  • Schweiger, H. G., 1969: Cell biology ofAcetabularia. Current Topics in Microbiology and Immunology. Berlin-Heidelberg-New York: Springer-Verlag, in preparation.

    Google Scholar 

  • andS. Berger, 1964: DNA-dependent RNA synthesis in chloroplasts ofAcetabularia. Biochim. Biophys. Acta87, 533–535.

    PubMed  Google Scholar 

  • undH. J. Bremer, 1961: Cytoplasmatische RNA-Synthese in kernlosen Acetabularien. Biochim. Biophys. Acta51, 50–59.

    PubMed  Google Scholar 

  • W. L. Dillard, A. Gibor, andS. Berger, 1967: RNA-Synthesis inAcetabularia. I. RNA-Synthesis in enucleated cells. Protoplasma64, 1–12.

    PubMed  Google Scholar 

  • Spencer, D., 1965: Protein synthesis by isolated spinach chloroplasts. Arch. Biochem. Biophys.111, 381–390.

    PubMed  Google Scholar 

  • , andP. Whitfeld, 1966: The nature of the ribonucleic acid of isolated chloroplasts. Arch. Biochem. Biophys.117, 337–346.

    PubMed  Google Scholar 

  • Stutz, E., andH. Noll, 1967: Characterization of cytoplasmic and chloroplast polysomes in plants: Evidence for three classes of ribosomal RNA in nature. Proc. nat. Acad. Sci. (U.S.A.)57, 774–781.

    Google Scholar 

  • Werz, G., andG. Kellner, 1968: Molecular characteristics of chloroplast DNA ofAcetabularia cells. J. Ultrastruct. Res.24, 109.

    PubMed  Google Scholar 

  • Wilkins, M. H. F., 1963: Molecular configuration of nucleic acids. Science140, 941–950.

    PubMed  Google Scholar 

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Dillard, W.L., Schweiger, H.G. RNA synthesis inAcetabularia . Protoplasma 67, 87–100 (1969). https://doi.org/10.1007/BF01256769

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  • DOI: https://doi.org/10.1007/BF01256769

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