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A method for recording the circadian rhythm of the oxygen balance in a single cell ofAcetabularia mediterranea

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Summary

A polarographic method using a platinum electrode permits continuous recording of the oxygen balance of a singleAcetabularia cell during several days. The amount of oxygen which is consumed by the electrode is immeasurably small. The accuracy of the method is definitely better than 0.05Μl O2/ml medium. The resolution in time is less than 5 min. The length of one period in the circadian rhythm of photosynthesis inAcetabularia has been found to be about 27 hours.

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References

  • Gleichmann, U., undD. W. Lübbers, 1960: Die Messung des Sauerstoffdruckes in Gasen und Flüssigkeiten mit der Pt-Elektrode unter besonderer Berücksichtigung der Messung im Blut. Pflügers Arch. ges. Physiol. Menschen, Tiere271, 431–455.

    Google Scholar 

  • HÄmmerling, J., 1963: Nucleo-cytoplasmic interactions inAcetabularia and other cells. Ann. Rev. Plant Phys.14, 65–92.

    Google Scholar 

  • Schweiger, E., H. G. Wallraff undH. G. Schweiger, 1964: über tagesperiodische Schwankungen der Sauerstoffbilanz kernhaltiger und kernloserAcetabularia mediterranea. Z. Naturforsch.19 b, 499–505.

    Google Scholar 

  • Sweeney, B. M., 1960: The photosynthetic rhythm in single cells ofGonyaulax polyedra. Cold Spring Harbor Symposia on Quant. Biol.25, 145–148.

    Google Scholar 

  • — andF. Haxo, 1961: Persistence of a photosynthetic rhythm in enucleatedAcetabularia. Science134, 1361–1363.

    Google Scholar 

  • Terborgh, J., 1966: Potentiation of photosynthetic oxygen evolution in red light by small quantities of monochromatic blue light. Plant Physiol.41, 1401–1410.

    Google Scholar 

  • — andG. C. McLeod, 1967: The photosynthetic rhythm ofAcetabularia crenulata, I. Continuous measurements of oxygen exchange in alternating light-dark regimes and in constant light of different intensities. Biol. Bull.133, 659–669.

    Google Scholar 

  • Vanden Driessche, T., 1966: Circadian rhythms inAcetabularia: Photosynthetic capacity and chloroplast shape. Exp. Cell Res.42, 18–30.

    PubMed  Google Scholar 

  • Zeuthen, E., 1955: Mitotic respiratory rhythms in single eggs ofPsammechinus miliaris and ofCiona intestinalis. Biol. Bull.108, 366–385.

    Google Scholar 

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Author notes

  1. H. G. Schweiger

    Present address: Max-Planck-Institut für Zellbiologie, Wilhelmshaven, Deutschland

Authors and Affiliations

  1. Max-Planck-Institut für Zellbiologie, Wilhelmshaven, Deutschland

    L. von Klitzing

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  1. L. von Klitzing
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  2. H. G. Schweiger
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von Klitzing, L., Schweiger, H.G. A method for recording the circadian rhythm of the oxygen balance in a single cell ofAcetabularia mediterranea . Protoplasma 67, 327–332 (1969). https://doi.org/10.1007/BF01254898

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  • DOI: https://doi.org/10.1007/BF01254898

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