Summary
The effect of several environmental factors on the susceptibility of primary cultures of porcine kidney (PK) cells to infection with foot-and-mouth disease (FMD) virus was determined. Cell susceptibility was affected by initial pH of growth and maintenance media and by concentration of cells used to prepare cultures. Plaque formation was affected by composition of fluid in which the virus was diluted. Yield of virus was affected by the composition of culture fluid during replication and release of virus. Cells in cultures prepared by the methods described were highly susceptible to strains of the 7 types of FMD virus obtained directly from animals. A mean of more than 1,000 plaque-forming units (PFU) of FMD virus per cell was obtained from cultures prepared as described for replication and assay of these strains of virus on second passage in cultured cells. Although dispersed cells were centrifuged to remove trypsin, cultures of high susceptibility to infection with FMD virus were prepared from dispersed cells that were not centrifuged.
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Patty, R.E. Methods for increasing the susceptibility of primary cultures of porcine kidney cells to infection with foot-and-mouth disease virus. Archiv f Virusforschung 33, 356–363 (1971). https://doi.org/10.1007/BF01254692
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DOI: https://doi.org/10.1007/BF01254692