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A polymer two phase system adapted to virus detection

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Summary

An aqueous polymer two phase concentration method has been tested as an aid to detection of five enteroviruses and of influenza virus type A. The system comprises dextran T-500-0.25%, polyethylene glycol 6000-7.0% and NaCl-0.3m. Fecal solids, when present, are best removed by centrifugation before the polymers are added. After 18 to 24 hours in the cold, the suspension has separated to yield a small bottom phase which has about 2% of the original sample volume and nearly half of the virus. Compared to virus in a standard laboratory diluent, virus in this bottom phase might be slightly stimulated or mildy inhibited in its infectivity for primary monkey kidney cell cultures. In contrast to previous experience with another two phase concentration system, none of the inhibited virus types was affected to such a degree that the treatment was inadvisible.

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References

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Author notes

  1. D. O. Cliver

    Present address: Food Research Institute and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, USA

Authors and Affiliations

  1. Food Research Institute and Department of Bacteriology, University of Wisconsin, Madison, Wisconsin, USA

    Jean Grindrod

Authors
  1. Jean Grindrod
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  2. D. O. Cliver
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Grindrod, J., Cliver, D.O. A polymer two phase system adapted to virus detection. Archiv f Virusforschung 31, 365–372 (1970). https://doi.org/10.1007/BF01253770

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  • DOI: https://doi.org/10.1007/BF01253770

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