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Viral auto-inhibition studied by the effect of anti-interferon serum on plaque formation

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Summary

Semliki Forest Virus (SFV), Newcastle Disease Virus (NDV) and Vesicular Stomatitis Virus (VSV) were selected on account of their capacity to induce and to be affected by interferon. They were tested in mouse L cells by the plaque method with, or without the addition of mouse anti-interferon serum (MAIS) into the nutrient ‘Agarose’ layer.

Only SFV plaques were greatly enlarged by MAIS. The number of enlarged plaques was augmented by increased amounts of MAIS. When high doses of MAIS were used, SFV plaques were observed to be of approximately the same size and were all large. This result suggests that smallest SFV plaques were produced by virus particles that induced more interferon than did others, since the highest concentrations of MAIS were necessary for their maximal development.

NDV plaques size was slightly increased by MAIS, indicating that the virus was a little sensitive to interferon. Abortive replication of the virus in L cells could not, however, be related to a possible auto-inhibiting phenomenon.

When a mixed population of large plaque and small plaque variants of VSV was grown with MAIS, only large plaques were obtained. This data supports the view that VSV small plaque variant produces interferon and can be considered a self-inhibiting virus.

With these results, conditions for virus plaque enhancement by MAIS were defined.

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  1. B. Fauconnier

    Present address: Institut Pasteur, Paris, France

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    1. B. Fauconnier
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    Fauconnier, B. Viral auto-inhibition studied by the effect of anti-interferon serum on plaque formation. Archiv f Virusforschung 31, 266–272 (1970). https://doi.org/10.1007/BF01253761

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    • DOI: https://doi.org/10.1007/BF01253761

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