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A comparison of two methods of investigating sodium uptake by bean-leaf cells and the vitality of isolated leaf-cells

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Summary

Net sodium fluxes into thin bean-leaf sections were 65 times higher than fluxes into enzymatically isolated cells. Isolated mesophyll cells did not stain with neutral red, the dye was, however, accumulated by similar cells in small pieces of tissue which had not been separated by the enzymatic treatment. It is concluded that the plasmatic membranes of isolated bean-leaf cells are damaged and, hence, unfit for studies of ion. uptake.

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Author notes

  1. B. Jacoby

    Present address: Department of Plant Physiology, The Hebrew University, Rehovot, Israel

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  1. Department of Plant Physiology, The Hebrew University, Rehovot, Israel

    J. Dagan

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  1. B. Jacoby
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  2. J. Dagan
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Jacoby, B., Dagan, J. A comparison of two methods of investigating sodium uptake by bean-leaf cells and the vitality of isolated leaf-cells. Protoplasma 64, 325–329 (1967). https://doi.org/10.1007/BF01253710

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  • DOI: https://doi.org/10.1007/BF01253710

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