Summary
A coupled peroxidation technique for localization of monoamine oxidase (MAO-A and MAO-B), applied to post-mortem fixed tissue of the locus coeruleus of the Mongolian gerbil is demonstrated. Tyramine hydrochloride,Β-phenylethylamine and 5-hydroxytryptamine creatinine sulphate were used as substrates, l-deprenyl and clorgyline served as specific inhibitors. All three substrates stained the neurons of locus coeruleus in the absence of inhibitor. In the presence of 1-deprenyl, tyramine hydrochloride and 5-hydroxytryptamine creatinine sulphate were metabolized, whereas in the presence of clorgyline no reaction with either substrate could be observed. Immunocytochemical staining of tyrosine hydroxylase (TH) was employed as comparison.
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Konradi, C., Jellinger, K., Riederer, P. et al. Histochemistry of MAO-A and MAO-B in the locus coeruleus of the Mongolian gerbil. J. Neural Transmission 70, 369–376 (1987). https://doi.org/10.1007/BF01253611
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DOI: https://doi.org/10.1007/BF01253611