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Separation of ECHO virus type 6 antigens by centrifugation in caesium chloride density gradients

I. Infectivity and antigenic activities

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Summary

N and H antigens of ECHO virus 6 were separated by caesium chloride density gradient centrifugation. The N particles came to equilibrium at a buoyant density of 1.33 g/ml, this band carrying peak infectivity, precipitating activity of N character and complement-fixing antigenicity. The H particles equilibrated at a buoyant density of 1.30 g/ml, this band carrying precipitating activity of H character and high complement-fixing antigenicity but, presumably as a contaminant, lower infectivity. Heated virus material (30 minutes at 56° C) equilibrated at a buoyant density equal to that of the naturally occurring H antigen. Quantitative data of infectivity, complement-fixing and precipitating activities are discussed.

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  1. Marianne Forsgren

    Present address: Virus Department of the Central Bacteriological Laboratory of Stockholm City, Stockholm, Sweden

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    1. Marianne Forsgren
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    Forsgren, M. Separation of ECHO virus type 6 antigens by centrifugation in caesium chloride density gradients. Archiv f Virusforschung 28, 19–25 (1969). https://doi.org/10.1007/BF01250841

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    • DOI: https://doi.org/10.1007/BF01250841

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