Summary
The influence of complement on the neutralization of cytomegalovirus by antibodies has been studied using human sera and antisera prepared in rabbits with various strains of cytomegalovirus (Ad 169, C 87, Davis andT 27). Examination of patient sera showed that addition of 2 per cent complement had only a weak and non-significant enhancing effect on neutralization, even by sera taken early in the course of infection.
It proved possible to produce specific antisera with high titres in rabbits with the four strains of cytomegalovirus. It was found that addition of 2 per cent complement had an enhancing effect on neutralization of cytomegalovirus by hyperimmune sera. Concerning the Ad 169 and C 87 antisera the effect of complement was essentially limited to early antisera, whereas the effect was especially pronounced on late Davis and T 27 antisera. The late Ad 169 and C 87 sera did not require complement, whereas all late Davis and T 27 antisera were complement-requiring. These differences, which most likely are bound to whether the anti bodies are γG or γM antibodies make it important to use complement in cross-neutralization experiments with hyperimmune sera.
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Andersen, H.K. The influence of complement on cytomegalovirus neutralization by antibodies. Archiv f Virusforschung 36, 133–140 (1972). https://doi.org/10.1007/BF01250303
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DOI: https://doi.org/10.1007/BF01250303