Skip to main content
SpringerLink
Log in

Cytotoxicity induced by mixtures of adenovirus and specific antibodies

Induction of cytotoxic aggregates by homotypic anti-fibre sera and neutralization of the cytotoxicity by homotypic anti-hexon sera

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

Aggregates cytotoxie for human cellsin vitro were produced when adenovirus type 5 was mixed with homotypic rabbit antiserum against whole virus particles at a certain restricted virus-antibody ratio. The same degree of cytotoxicity was obtained with mixtures of the virus and an antiserum against purified fibres of adenovirus type 5 at a certain proportion. The cytotoxicity could be neutralized by homotypic antisera against purified hexons. The sequence in which the two anti-capsomer sera were added to the virus was found to be of importance: complete neutralization of the cytotoxicity appeared when virus preparations were incubated with anti-hexon sera before cytotoxic aggregates were formed by antifibre sera. In this case even a significantly lower51Cr-release appeared than that produced by corresponding amounts of virus alone. No neutralization of the cytotoxicity was obtained when anti-hexon serum was added to the mixtures after formation of cytotoxic aggregates by anti-fibre antibodies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ankerst, J., andH. O. Sjögren: Cross-reacting TSTAs in adeno 7 and 12 tumors demonstrated by51Cr-cytotoxicity and isograft rejection test. Int. J. Cancer4, 279–287 (1969).

    Google Scholar 

  • Kjellén, L.: Cytotoxicity by antigen aggregation. Adenovirus neutralization assays as a model. Arch. ges. Virusforsch.39, 1–12 (1972).

    Google Scholar 

  • Kjellén, L., and J.Ankerst: Cytotoxicity of adenovirus-antibody complexes. Specificity of antibody and virus-antibody ratio at which cytotoxicity is induced. Europ. J. Immunol. In press. (1973).

  • Norrby, E.: The relationship between the soluble antigens and the virion of adenovirus type 3. IV. Immunological complexity of soluble components. Virology37, 565–576 (1969).

    Google Scholar 

  • Norrby, E., andY. Gollmar: Mosaics of capsid components produced by cocultivation of certain human adenovirusesin vitro. Virology44, 599–608 (1971).

    Google Scholar 

Download references

Author information

Author notes

  1. J. Ankerst

    Present address: Institute of Medical Microbiology, Department of Virology, University of Lund, Lund, Sweden

Authors and Affiliations

  1. Institute of Medical Microbiology, Department of Virology, University of Lund, Lund, Sweden

    L. Kjellén

Authors
  1. J. Ankerst
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Kjellén
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ankerst, J., Kjellén, L. Cytotoxicity induced by mixtures of adenovirus and specific antibodies. Archiv f Virusforschung 41, 99–105 (1973). https://doi.org/10.1007/BF01249934

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01249934

Keywords

Search

Navigation