Skip to main content
SpringerLink
Log in

Effect of enhancer on growth cycle of ECHO virus 6 plaque mutant

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

Comparison of the growth pattern of them + mutant of ECHO virus 6 in its permissive (human) and restrictive (monkey) hosts indicated that defects in monkey cells were expressed as low efficiency of plaquing, prolonged eclipse period and retarded release of virus. Simultaneous addition of virus and a subcellular fraction obtained from permissive cells (enhancer) removed these growth defects ofm + virus in monkey cells. Increasing the input multiplicity could correct only part of the defect, -i.e., shorten the eclipse period. Addition of enhancer during the eclipse period (0.5 to 5.0 hours) accelerated release of virus, but did not enhance efficiency of plaquing. Studies of events early in the growth cycle suggested that virus could be enhanced only prior to attachment. Although the mechanism has not been elucidated, the results suggest that enhancer may facilitate entry as well as exit ofm + virus grown in restrictive cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Suto, T., D. T. Karzon, andK. H. Bussell: Studies of mutants of ECHO virus 6. III. Behavior in monkey and human cell culture. Amer. J. Epidem.81, 341–349 (1965).

    Google Scholar 

  2. Righthand, F., andD. T. Karzon: Enhanced efficiency of ECHO virus 6 by a transferable factor in cultured human cells. Proc. Soc. exp. Biol. (N.T.)124, 1248–1254 (1967).

    Google Scholar 

  3. Barron, A. L., andD. T. Karzon: Studies of mutants of ECHO virus 6. I. Biologic and serologic characteristics. Amer. J. Epidem.81, 323–332 (1965).

    Google Scholar 

  4. Liu, O. C., andW. Henle: Studies on host-virus interactions in the chick embryo-influenza virus system. V. Simultaneous serial passage of the agents of influenza A and B in relation to variations in the growth cycle of influenza B virus. J. exp. Med.94, 291–304 (1951).

    Google Scholar 

  5. Dulbecco, R., andM. Vogt: One-step growth curve of western equine encephalomyelitis virus on chicken embryo cells grownin vitro and analysis of virus yields from single cells. J. exp. Med.99, 183–199 (1954).

    Google Scholar 

Download references

Author information

Author notes

  1. F. Righthand

    Present address: Department of Microbiology, School of Medicine, Wayne State University, 48207, Detroit, MI

  2. D. T. Karzon

    Present address: Department of Pediatrics, School of Medicine, Vanderbilt University, 37203, Nashville, TN

Authors and Affiliations

  1. Departments of Pediatrics and Microbiology, School of Medicine, State University of New York at Buffalo, New York, USA

    F. Righthand & D. T. Karzon

Authors
  1. F. Righthand
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. T. Karzon
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by research grant (AI-2396) and training grant (AI-98) from the National Institute of Allergy and Infectious Diseases.

Recipient of Research Career Award (AI-1136) from the National Institute of Allergy and Infectious Diseases.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Righthand, F., Karzon, D.T. Effect of enhancer on growth cycle of ECHO virus 6 plaque mutant. Archiv f Virusforschung 27, 290–300 (1969). https://doi.org/10.1007/BF01249651

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01249651

Keywords

Search

Navigation