Summary
Previously we were able to isolate by simple and mild methods from an aqueous sheep pineal extract an inhibiting principle acting on the anterior hypophysis of male ratsin vitro. This substance could be located by paper electrophoresis. In the present paper we describe the further purification of this active principle by paper chromatography in two different solvents. In two regions possessing a special fluorescence an inhibiting factor of the gonadotropic activity of the anterior pituitary was foundin vitro.
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Balemans, M. G. M., Ebels, I., Vonk-Visser, D. M. A.: Separation of pineal extracts on Sephadex G-10. I. A spectrofluorimetric study of indoles in a cockerel pineal extract. J. Neuro-Visc. Rel.32, 65–73 (1970).
Bertrand, D.: sFluorescence visible et constitution chimique. VI. Étude de quelques dérivés de l'indole et de la carboline. Bull. Soc. Chim.12, 1029–1033 (1945).
Ebels, I., Balemams, M. G. M., Verkleij, A. J.: Separation of pineal extracts on Sephadex G-10. II. A spectrofluorimetric and thinlayer chromatographie study of indoles in a sheep pineal extract. J. Neuro-Visc. Rel.32, 270–282 (1972).
Ebels, I., Benson, B.,Matthews, M. J.: Localization of a sheep pineal anti-gonadotropin. Anal. Biochem.56, 546–565 (1973).
Ebels, I., Citharel, A., Moszkowska, A.: Separation of pineal extracts by gelfiltration. III. Sheep pineal factors acting either on the hypothalamus, or on the anterior hypophysis of mice and rats in in vitro experiments. J. Neural Transm.36, 281–302 (1975).
Hess, S. M., Udenfriend, S.: A fluorimetric procedure for the measurement of tryptamine in tissues. J. Pharmacol. Exptl. Therap.127, 175–177 (1959).
Kidder, G. W., Deweij, V. C.: A new pteridine from Tetrahymena. J. Biol. Chem.243, 826–833 (1968).
Moszkowska, A., Ebels, I.: A study of the antigonadotropic action of synthetic arginine vasotocin. Experientia24, 610–611 (1968).
Moszkowska, A., Ebels, I.: The influence of the pineal body on the gonado-tropic function of the hypophysis. J. Neuro-Visc. Rel., Suppl. X, 160 to 176 (1971).
Moszkowska, A., Citharel, A., L'Héritier, A., Ebels, I., Laplante, E.: Some new aspects of a sheep pineal gonadotropic inhibiting activity in in vitro experiments. Experientia30, 964–995 (1974).
Niswender, G. D., Rees Midgley, A., Jr., Monroe, S. E., Reichert, L. E., Jr.: Radioimmunoassay for rat luteinizing hormone with antiovine LH serum and ovine LH-13J. Proc. Soc. Exp. Biol. Med. (N.Y.)128, 807 to 811 (1968).
Zurburg, W., Ebels, I.: Separation of pineal extracts by gelfiltration. I. Isolation from sheep pineals of a substance with special fluorescence characteristics. J. Neural Transm.35, 117–124 (1974).
Zurburg, W., Ebels, I.: Separation of pineal extracts by gelfiltration. II. Identification and isolation of two indoles from sheep pineal glands. J. Neural Transm.36, 59–69 (1975).
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This paper is dedicated to Prof. Dr.W. Birkmayer on the occasion of his 65th birthday.
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Moszkowska, A., Hus-Citharel, A., L'Héritier, A. et al. Separation of pineal extracts by gelfiltration V. location by paper chromatography of a sheep pineal principle inhibiting hypophyseal gonadotropic activity. J. Neural Transmission 38, 239–247 (1976). https://doi.org/10.1007/BF01249441
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DOI: https://doi.org/10.1007/BF01249441