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Glutaraldehyde as a fixative for plant tissues

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Summary

  1. 1.

    The use of glutaraldehyde (GDH) as a fixative for plant tissue is described and illustrated.

  2. 2.

    The effects of pH, concentration and combination with heavy metals on fixation are evaluated.

  3. 3.

    Fixation with a buffered 4% solution at pH 4.0 resulted in very good preservation of tissue and cell morpholgy.

  4. 4.

    The use of GDH fixation for certain histochemical tests is evaluated.

  5. 5.

    It is concluded that GDH is a useful fixative for plant tissue for a wide range of studies.

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Author notes

  1. M. Rubio-Huertos

    Present address: Instituto „Jaime Ferrán“ deham 15, England

Authors and Affiliations

  1. Department of Botany, University of Birmingham, England

    G. I. Moss

Authors
  1. G. I. Moss
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  2. M. Rubio-Huertos
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Moss, G.I., Rubio-Huertos, M. Glutaraldehyde as a fixative for plant tissues. Protoplasma 62, 194–200 (1966). https://doi.org/10.1007/BF01248082

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  • DOI: https://doi.org/10.1007/BF01248082

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