Summary
Amaranthus plants infected with a virus of rod-shaped particles showed under the light microscope intracytoplasmic amorphous and crystalline inclusions.
The submicroscopic organization of mesophyll cells from infectedAmaranthus leaves by electron microscopy is described. Besides big crystalline inclusions, long dark inclusions correspondent to needle-like inclusions observed by light microscopy are definable in the cytoplasm. The amorphous inclusion bodies were formed by an overgrown protrusion of vacuolate cytoplasm containing virus particles, long very dark stained inclusions forming dense bands and rings, normal elements of the cytoplasm such as mitochondria, endoplasmic reticulum and ribosomes, and some spherosomes. Inclusions and virus particles were not found in chloroplasts, mitochondria or nuclei of infected cells.
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References
Brandes, J., 1957: Eine elektronenmikroskopische Schnellmethode zum Nachweis faden- und stäbchenförmiger Viren, insbesondere in Kartoffeldunkelkeimen. Nachrichtenbl. dtsch. Pflanzenschutzd. 151–152. Braunschweig.
Brenner, S., and R. W. Horne, 1959: A negative staining method for high resolution electron microscopy of viruses. Biochim. Biophys. Acta34, 103–110.
Frey-Wyssling, A., and K. Mühlethaler, 1965: Ultrastructural plant cytology. Elsevier ed.
Goldin, M., 1960: Investigation of Tobacco Mosaic virus in ultrathin sections. Virology10, 4, 538–542.
Hayashi, T., C. Matsui, and A. Yamaguchi, 1965: Electron microscopy of intracellular turnip mosaic virus. Phytopathology55, 458–461.
Kolehmainen, L., H. Zech, and D. von Wettstein, 1965: The structure of cells during Tobacco Mosaic virus reproduction. J. Cell. Biol.25, 77–99.
Matsui, C., and A. Yamaguchi, 1964: Electron microscopy of host cells infected with tobacco etch virus. I. Virology22, 40–47.
Rubio-Huertos, M., 1950: Estudios sobre inclusiones intracellulares producidas por virus en las plantas. Microbiol. Española3, 207–232.
—, 1962: Light and electron microscopy of inclusion bodies associated with Petunia Ringspot virus. Virology18, 337–342.
—, 1965: Golgi apparatus hypertrophy associated with Petunia ringspot virus infection (Previous communication). Microbiol. Española18, 1–3.
—, and F. Garcia Hidalgo, 1964: Ultrathin sections of intranuclear and intracytoplasmic inclusions induced by Severe etch virus. Virology24, 84–90.
Shalla, T. A., 1964: Assembly and aggregation of Tobacco mosaic virus in tomato leaflets. J. Cell Biol.21, 253–264.
Zech, H., 1960: Intermediary products formed during TMV reproduction. Virology11, 499.
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Rubio-Huertos, M., Vela-Cornejo, A. Light and electron microscopy of virus inclusions inAmaranthus lividus cells. Protoplasma 62, 184–193 (1966). https://doi.org/10.1007/BF01248081
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DOI: https://doi.org/10.1007/BF01248081