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Co-localization of the β-subtype of protein kinase C and phosphorylation-dependent immunoreactivity of neurofilaments in intact, decentralized and axotomized rat peripheral neurons

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Journal of Neurocytology

Summary

The localizations of protein kinase C-β-immunoreactivity and phosphorylation-dependent immunoreactivity of neurofilaments were compared in rat dorsal root, hypogastric, and superior cervical ganglia. In all the ganglia studied, protein kinase C-β and phosphorylation-dependent immunoreactivity of neurofilaments were co-localized in nerve fibres, and no fibres with only protein kinase C-β-immunoreactivity or phosphorylation-dependent immunoreactivity of neurofilaments were observed. Most intense perikaryal protein kinase c-β and phosphorylation-dependent neurofilament-staining were seen in large dorsal root ganglion neurons, whereas in the superior cervical ganglion only very faint protein-kinase C-β and no phosphorylation-dependent staining was seen in the neuronal cell bodies. Both decentralization and axotomy of the superior cervical ganglion induced an accumulation of protein-kinase C-β-immunoreactivity and phosphorylation-dependent immunoreactivity of neurofilaments in the majority of neuronal perikarya. The accumulation was first observed at 1–2 days postoperation and it persisted up to 6–10 days postoperation. In strongly labelled decentralized neuronal perikarya, precipitation of immunoreactivity was seen near the cell and nuclear membranes, whereas in axotomized neurons, immunoreactivity was often concentrated as a unipolar clump in the cytoplasm. The results show that protein kinase C-β-immunoreactivity and phosphorylation-dependent immunoreactivity of neurofilaments are colocalized in intact rat peripheral ganglia and that both accumulate transiently in cell bodies of the superior cervical ganglion after decentralization and axotomy.

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Authors and Affiliations

  1. Department of Public Health, University of Tampere, 33101, Tampere, Finland

    R. Roivainen

  2. Department of Biomedical Sciences, University of Tampere, 33101, Tampere, Finland

    S. T. Nikkari

  3. Laboratory of Neurobiology and Anesthesiology, National Institute of Dental Research, NIH, Bethesda, MD, USA

    M. J. Iadarola

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  1. R. Roivainen
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  2. S. T. Nikkari
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  3. M. J. Iadarola
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  4. J. Koistinaho
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Roivainen, R., Nikkari, S.T., Iadarola, M.J. et al. Co-localization of the β-subtype of protein kinase C and phosphorylation-dependent immunoreactivity of neurofilaments in intact, decentralized and axotomized rat peripheral neurons. J Neurocytol 22, 154–163 (1993). https://doi.org/10.1007/BF01246354

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  • DOI: https://doi.org/10.1007/BF01246354

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