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Topography and Morphometric Characteristics of NF200+ Neurons in the Gray Matter of the Spinal Cord after Capsaicin Deafferentation

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Experiments were performed on 80 white male Wistar rats aged 3, 10, 20, 30, 60, 180, and 360 days to address the influences of capsaicin deafferentation on the topography of neurons containing 200-kDal neurofilament protein (NF200+) in the gray matter of the second thoracic segment of the spinal cord. Deafferentation was modeled by s.c. administration of single doses of 150 mg/kg capsaicin to rat pups on day 2 of life. Controls were intact animals (n = 40). NF200+ neurons were detected by labeling cells with anti-NF200 antibodies. The proportion of immunoreactive neurons was calculated by labeling the whole neuron population with NeuroTrace Green Fluorescent Nissl Stain. The results obtained from these experiments show that NF200+ neurons were detected in the ventral horn, the intermediate zone, and the dorsal horn of the spinal cord and were discriminated by their morphometric characteristics. Administration of capsaicin led to identical decreases in the numbers and mean cross sectional areas of medially and laterally located NF200+ motoneurons in the spinal cord (plate IX). In the intermediate zone (plate VII) and dorsal horn (plates III–IV), there were no changes in the numbers of NF200+ neurons, though the mean cross sectional area was greater than that in controls.

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Authors and Affiliations

  1. Department of Normal Physiology with Biophysics (Director: Professor P. M. Maslyukov), Yaroslavl State Medical Academy, 5 Revolyutsionnaya Street, 15000, Yaroslavl, Russia

    V. V. Porseva

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  1. V. V. Porseva
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Correspondence to V. V. Porseva.

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Translated from Morfologiya, Vol. 144, No. 6, pp. 20–25, November–December, 2013.

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Porseva, V.V. Topography and Morphometric Characteristics of NF200+ Neurons in the Gray Matter of the Spinal Cord after Capsaicin Deafferentation. Neurosci Behav Physi 44, 919–923 (2014). https://doi.org/10.1007/s11055-014-0003-6

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  • DOI: https://doi.org/10.1007/s11055-014-0003-6

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