Summary
In this study we have examined brain concentrations of monoamine neurotransmitters and striatal and mesencephalic D-2 receptors in a chronic model of herpes simplex virus (HSV) encephalitis. The HSV-inoculated rabbits were killed two months after inoculation. Dopamine (DA), noradrenaline, serotonin and their metabolites were determined in the substantia nigra, caudate nucleus, putamen, nucleus accumbens, and olfactory tubercles using HPLC with electrochemical detection. The Bmax and Kd values of D-2 receptors were studied in the striatum and in the mesencephalon using3H-spiroperidol as ligand.
The animals showed rotational behaviour, consisting of posture tilting to the inoculated side and circling in the same direction during the first week, then slowly subsiding. Compared with controls, the concentration of homovanillic acid (HVA) was reduced in the ascending DA system on both sides. Neither in the number nor affinity of D-2 receptors were there any differences between the HSV-inoculated and control rabbits.
The decreased HVA concentrations suggest that dopaminergic hypofunction can develop as a consequence of previously experienced acute HSV brain infection.
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Deatly AM, Spivack JG, Lavi E, O'Boyle DR II, Fracer NW (1988) Latent herpes simplex virus type 1 transcripts in periferal and central nervous system tissues of mice map to similar regions of the viral genome. J Virol 62: 749–756
Dixon WJ (1985) (chief ed) BMDP Statistical Software. University of California Press, Berkeley
Duvoisin RC, Yahr MD (1965) Encephalitis and parkinsonism. Arch Neurol 12: 227–239
Kataoka K, Bak I, Markham CH (1979) Alterations in tyrosine hydroxylase, glutamic acid decarboxylase and choline acetyltransferase in basal ganglia following herpes simplex virus inoculation in rat neostriatum. Brain Res 169: 401–405
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Lycke E, Roos B-E (1972) The monoamine metabolism in viral encephalitides of the mouse. II. Turnover of monoamines in mice infected with herpes simplex virus. Brain Res 44: 603–613
Lycke E, Roos B-E (1974) Influence of changes in brain monoamine metabolism on behaviour of herpes simplex-infected mice. J Neurol Sci 22: 277–289
Lycke E, Roos B-E (1975) Virus infections in infant mice causing persistent impairment of turnover of brain catecholamines. J Neurol Sci 26: 49–60
Lycke E, Modigh K, Roos B-E (1970) The monoamine metabolism in viral encephalitides of the mouse. I. Virological and biochemical results. Brain Res 23: 235–246
McPherson GA (1985) Analysis of radioligand binding experiments: a collection of computer programs for the IBM PC. J Pharmacol Methods 14: 213–228
Neeley SP, Cross AJ, Crow TJ, Johnson JA, Taylor GR (1985) Herpes simplex virus encephalitis. Neuroanatomical and neurochemical selectivity. J Neurol Sci 71: 325–337
Price RW, Rubenstein R, Joh TH, Reis RJ (1981) Tyrosine hydroxylase activity in the superior cervical ganglion during HSV infection: correlation with viral titers and viral antigen. Brain Res 214: 357–370
Päivärinta MA, Marttila RJ, Koulu M, Pesonen U, Röyttä M, Rinne UK (1991) Brain monoamine metabolism and rotational behaviour induced by experimental herpes simplex virus encephalitis. J Neural Transm [GenSect] 86: 181–190
Päivärinta MA, Marttila RJ, Rinne JO, Rinne UK (1992) Decrease in mesencephalic dopamine autoreceptors in experimental herpes simplex encephalitis. J Neural Transm [GenSect] 89: 71–80
Seegal RF, McFarland DJ (1988) Stereotaxic microinjection of HSV-1 selectively decreases striatal dopamine concentrations in mice. Brain Res 445: 234–240
Seegal RF, Sikora E, Hotchin J (1980) Locomotor effects of catecholaminergic drugs on herpes-infected mice. Pharmacol Biochem Behav 12: 61–66
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Päivärinta, M.A., Marttila, R.J., Rinne, J.O. et al. Dopaminergic neurotransmission in chronic herpes simplex virus brain infection in rabbits. J. Neural Transmission 93, 205–212 (1993). https://doi.org/10.1007/BF01244997
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DOI: https://doi.org/10.1007/BF01244997