Abstract
In the present study electron probe X-ray microanalysis (EPMA) was used to examine the elemental contents in samples of biological molecules from aqueous solutions and of cultured cells. To preserve the contents of diffusible elements the freeze-drying technique was applied as a preparative procedure. The resulting powders were then formed into small tablets, weighing about 0.1 mg, which were analyzed with a wavelength-dispersive spectrometer (WDS). Using tablets, the total concentration of different elements was measured in biological substances isolated fromGalleria mellonella, and cells taken from a culture ofChlorella vulgaris treated with uranium nitrate.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J. I. Goldstein, D. E. Newbury, P. Echlin, D. C. Joy, C. Fiori, E. Lifshin,Scanning Electron Microscopy and X-ray Microanalysis, Plenum, New York, 1981.
P. Echlin,Low-temperature Microscopy and Analysis, Plenum, New York, 1992.
A. Sobota, I. V. Burovina, A. G. Pogorelov, A. A. Solus,Histochemistry 1984,81, 201.
N. A. Spiridonov, M. A. Kostenko, S. Volkova, A. G. Pogorelov, M. N. Kondrashova,Comp. Biochem. Physiol. 1984,78C, 207.
I. Wildhaber, H. Gross, H. J. Moor,Ultrastruct. Res. 1982,80, 367.
A. Pogorelov, B. Allachverdov, I. Burovina, G. Mazay, V. Pogorelova,J. Microsc. 1991,162, 255.
S. A. Smirnova, O. G. Scotnikova, B. L. Allachverdov, A. G. Pogorelov,Biological Sciences 1989,4, 42 (Russian).
A. Pogorelov,Micron Microsc. Acta 1987,18, 159.
T. A. Hall, in:Physical Techniques in Biological Research (G. E. Oster, ed.), Academic Press, New York, 1971, pp. 157–275.
Zs-Nagy, C. Pieni, C. Guili, C. Bertoni-Freddari, V. Zs-Nagy,J. Ultrastruct. Res. 1977,58, 22.
A. G. Pogorelov, B. L. Allachverdov,Micron. Microsc. Acta 1984,15, 177.
C. P. Lechene, in:Microprobe Analysis as Applied to Cells and Tissue (T. Hall, P. Echlin, R. Kaufmann, eds.), Academic Press, London, 1974, pp. 351–367.
T. Ichinokawa, H. Kobayashi, M. Nakajima,Jp. J. Appl. Phys. 1969,8, 1563.
N. A. Spiridonov, E. V. Kashparova, B. P. Baskunov,Comp. Biochem. Physiol. 1992,102C, 199.
W. Z. W. Ngah, J. N. Smith,Xenobiotica 1983,13, 383.
S. A. Smironva, O. G. Scotnikova, B. L. Allachverdov, A. G. Pogorelov,Biological Sciences 1988,9, 64 (Russian).
J. L. Oschman, B. L. Wall,J. Cell Biol. 1972,55, 58.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pogorelov, A., Pogorelova, V., Smirnova, S. et al. Electron probe microanalysis of dried biological substances. Mikrochim Acta 114, 405–411 (1994). https://doi.org/10.1007/BF01244567
Issue Date:
DOI: https://doi.org/10.1007/BF01244567