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Simultaneous monitoring of glutathione and major proteins in single erythrocytes

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Abstract

To gain more information from single-cell analysis, multiple detection schemes can be implemented in capillary electrophoresis. Red blood cells pre-treated with monobromobimane allow fluorescent tags to be attached to various thiol-containing compounds. Both derivatized glutathione and derivatized hemoglobin-A0 can be detected by observing emission at 495 nm when 275 nm excitation is used. At the same time, a second emission channel at 350 nm can be used to monitor the native fluorescence from carbonic anhydrase and from hemoglobin. Cross-correlation of the intracellular amounts of these provides additional insight into the cell-aging process.

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Authors and Affiliations

  1. Ames Laboratory-USDOE and Department of Chemistry, Iowa State University, 50011, Ames, IA, USA

    Kit-Sum Wong & Edward S. Yeung

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  1. Kit-Sum Wong
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  2. Edward S. Yeung
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Wong, KS., Yeung, E.S. Simultaneous monitoring of glutathione and major proteins in single erythrocytes. Mikrochim Acta 120, 321–327 (1995). https://doi.org/10.1007/BF01244442

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  • DOI: https://doi.org/10.1007/BF01244442

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