Skip to main content
SpringerLink
Log in

Removal of contaminating protein from high titre virus suspensions to be used in the production of antisera for immimofluorescence

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

A method is described for the removal of contaminating (endogenous) protein from high titre virus suspension. The method depends upon adsorption of the virus to barium sulphate suspension at 37° C and its subsequent elution at 4° C into sodium citrate solution.

The efficiency of protein removal varied from 90 to 98.9%, corresponding to a fall from 1400 to 150 μg/ml, and from 9500 to 110 μg/ml with Coxsackie B 4 and mumps viruses, respectively. Other viruses, for which intermediate figures were obtained included several myxoviruses, vaccinia, herpes simplex and respiratory syncytial virus.

Subsequent immunization of guinea-pigs with ‘protein-reduced’ virus suspensions confirmed the retention of viral immunogenicity after treatment in terms of complement-fixing, neutralizing and immunofluorescent antibody titres induced. All the treated viruses except respiratory syncytial virus produced an antiserum in guinea-pigs completely free from non-specific fluorescent when used without dilution in a homologous immunofluorescent test system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lowry, O. H., N. J. Rosebrough, A. L. Farr, andR. J. Randall: Protein measurement with the folin phenol reagent. J. biol. Chem.193, 265–272 (1951).

    Google Scholar 

  2. Smirnova, V. I., M. I.Choporova, and E. I.Matsyukevich: Obtaining parainfluenza hemagglutinins of high titre. In: Problems of Vaccine and Sera Production, Kiev. 11–16 (1968).

  3. Smirnova, V. J., T. M. Rybakova, M. I. Choporova, andE. I. Matsyukevich: Purification and concentration of parainfluenza virus. Acta virol.13, 242–243 (1969).

    Google Scholar 

  4. Sommerville, R. G.: A rapid and reliable immunofluorescent method for the identification of common infections of the skin. Arch. ges. Virusforsch.26, 97–104 (1969).

    Google Scholar 

  5. Spence, D., G. Kenny, A. R. McLure, D. E. MacFarlane, andR. G. Sommerville: The preparation of an antiserum to adenovirus group (hexon) antigen, to be used for immunofluorescent detection of infection with different adenoviruses. Arch. ges. Virusforsch.34, 340–345 (1971).

    Google Scholar 

Download references

Author information

Author notes

  1. R. G. Sommerville

    Present address: Virology Section, University Department of Microbiology, Belvidere Hospital, Glasgow E. 1., Scotland

Authors and Affiliations

  1. Virology Section, University Department of Microbiology, Belvidere Hospital, Glasgow E. 1., Scotland

    D. E. MacFarlane & A. R. McLure

Authors
  1. D. E. MacFarlane
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. R. McLure
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. G. Sommerville
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

MacFarlane, D.E., McLure, A.R. & Sommerville, R.G. Removal of contaminating protein from high titre virus suspensions to be used in the production of antisera for immimofluorescence. Archiv f Virusforschung 34, 346–350 (1971). https://doi.org/10.1007/BF01242981

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01242981

Keywords

Search

Navigation