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Methods for Quantification of Viruses

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Protocols for the Diagnosis of Pig Viral Diseases

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Abstract

Virus quantification is widely practised in both commercial and academic laboratories involved in research or production of viral vaccines, recombinant proteins, viral antigens, or antiviral agents. For this, the cell culture-based endpoint dilution assays are the most widely used methods. However, these infectivity assays are laborious, time consuming, and susceptible to failures due to the contamination of cells. With the advancement in science, a number of other methods based on chemical or physical principles have been developed for determining the viral load in a given sample. These methods include electron microscopy, hemagglutination assay, qPCR, flow cytometry, and serological assays such as ELISA. However, all of these methods have their own limitations and advantages associated with them and therefore one must be careful while selecting an appropriate method to determine the virus titer and interpretation of results. Here, we describe the theory and practical aspects of the most commonly used methods for virus quantification and their practical utility in the field of virology.

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Author information

Authors and Affiliations

  1. Division of Biological Products, ICAR-IVRI, Bareilly, Uttar Pradesh, India

    Mukesh Bhatt, Chris Einstein,  Kiran, Arfa Fayaz, Vishal Rai, Monu Karki, Ashok Kumar, Ajay Kumar Yadav & Kaushal Kishor Rajak

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  1. Mukesh Bhatt
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  6. Monu Karki
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  7. Ashok Kumar
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  9. Kaushal Kishor Rajak
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Editor information

Editors and Affiliations

  1. National Research Centre on Pig, Indian Council of Agricultural Research, Guwahati, Assam, India

    Rajib Deb

  2. National Research Centre on Pig, Indian Council of Agricultural Research, Guwahati, Assam, India

    Ajay Kumar Yadav

  3. National Research Centre on Pig, Indian Council of Agricultural Research, Guwahati, Assam, India

    Swaraj Rajkhowa

  4. College of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab, India

    Yashpal Singh Malik

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Bhatt, M. et al. (2022). Methods for Quantification of Viruses. In: Deb, R., Yadav, A.K., Rajkhowa, S., Malik, Y.S. (eds) Protocols for the Diagnosis of Pig Viral Diseases. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2043-4_3

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  • DOI: https://doi.org/10.1007/978-1-0716-2043-4_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2042-7

  • Online ISBN: 978-1-0716-2043-4

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