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Comparative studies on nucleic acid synthesis and virus-induced RNA polymerase activity in mammalian cells infected with certain arboviruses

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Summary

Variations in the metabolism of African green monkey (AGMK) and stable porcine kidney (PS) cells infected with certain arboviruses were investigated. Virus-directed RNA synthesis and RNA polymerase activity were stimulated in actinomycin-treated infected cells. The rate of3H-uridine incorporation into RNA in Western equine encephalitis (WEE) virus-infected AGMK cells reached a maximum at approximately 5 hours after infection. In contrast, the viral RNA synthesis induced by Japanese encephalitis (JE) or dengue virus did not increase appreciably during the first 15 hours, and a maximum was reached from 24 to 30 hours after infection. Cytoplasmic large- and small-particle fractions from cells infected with WEEV or JEV were found to catalyze the incorporation of 4 nucleoside triphosphates into acid-insoluble products. In WEEV-infected AGMK cells, the enzyme activity was associated almost solely with the small-particle fraction, whereas nuclear and large-particle fractions of JEV-infeeted cells still contained 15 to 30% of the total enzyme activity 24 hours after infection. Rapid inhibition of cellular DNA synthesis was observed 4 hours after infection, with each of the three kinds of arbovirus used. Heat-inactivated WEEV was unable to suppress host cell DNA synthesis appreciably, whereas infection by UV-irradiated virus did result in a clear inhibition of DNA synthesis. However, the effect was apparently less marked than that of the active WEE virus.

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  1. M. Takehara

    Present address: Department of Microbiology, Kobe University School of Medicine, Kobe, Japan

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    Takehara, M. Comparative studies on nucleic acid synthesis and virus-induced RNA polymerase activity in mammalian cells infected with certain arboviruses. Archiv f Virusforschung 34, 266–277 (1971). https://doi.org/10.1007/BF01242972

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