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Electron microscopy of Ibaraki virus

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Summary

Ibaraki virus propagated in BK and BHK-21 cell culture, was investigated by means of negative contrast and ultrathin section electron microscopy. In negative contrast preparations, the virus was shown to possess an icosahedral configuration. The virion measured about 55 mμ in diameter and had no envelope. The capsid of the virus probably consists of a single layer of 32 capsomeres, arranged in 5 ∶ 3 ∶ 2 symmetry. One or more virions enclosed in a pseudo-envelope were observed occasionally. In sections of infected culture cells, virus particles with electron dense cores were associated with intracytoplasmic viral matrices. Unenveloped core particles, as well as more complex membrane-bound forms were occasionally observed. Acquisition of a limiting unit membrane envelope appears to occur during release by budding from cell membranes. Obviously the virus does not multiply in the nucleus.

These findings provide additional evidence for the similarity of Ibaraki virus to bluetongue virus. The remarkably close relationship between Ibaraki and bluetongue viruses has led to the suggestion that these two viruses should be clasified within the bluetongue subgroup of the newly proposed diplornavirus group.

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  1. Y. Ito

    Present address: National Institute of Animal Health, Kodaira, Tokyo, Japan

Authors and Affiliations

  1. National Institute of Animal Health, Kodaira, Tokyo, Japan

    Y. Tanaka, Y. Inaba & T. Omori

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  1. Y. Ito
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  2. Y. Tanaka
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  3. Y. Inaba
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  4. T. Omori
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Ito, Y., Tanaka, Y., Inaba, Y. et al. Electron microscopy of Ibaraki virus. Archiv f Virusforschung 40, 29–46 (1973). https://doi.org/10.1007/BF01242633

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  • DOI: https://doi.org/10.1007/BF01242633

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