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Assay of measles virus hemolysin and its antibody

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Summary

An assay method of measles virus hemolysin (HL) was developed utilizing spectrophotometric measurement of released hemoglobin. The method was based on the linear relationship, in a certain range of HL dilution, between the percentage of hemolyzed erythrocytes and the HL dilution as expressed in a logarithmic scale, and a HL unit was defined. The hemolysis inhibition (HLI) by measles immune serum was represented by an S-shaped symmetric curve with a constant amount of HL when the per cent HLI was plotted against the serum dilution expressed in a logarithmic scale, and the HLI antibody titer was expressed by the serum dilution showing 50% inhibition. The results of HLI test on children, though preliminary in nature, seemed to show that the test is satisfactory enough for routine use. These assay methods will be obviously very useful in studies of measles, in particular enable us to perform rational studies of HL and its activity, which would in turn contribute to the understanding of the nature of the virus and its infection.

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References

  1. Periés, J. S., andC. Chany: Activité hémagglutinante et hémolytique du virus morbilleux. C. R. Acad. Sci.251, 820–821 (1960).

    Google Scholar 

  2. DeMeio, J. L.: Measles virus hemolysin. Virology16, 342–344 (1962).

    PubMed  Google Scholar 

  3. Norrby, E. C. J., andL. G. Falksveden: Some general properties of the measles virus hemolysin. Arch. ges. Virusforsch14, 474–486 (1964).

    PubMed  Google Scholar 

  4. Schluederberg, A. E.: Separation of measles virus particles in density gradients. Amer. J. Dis. Childr.103, 291–296 (1962).

    Google Scholar 

  5. Norrby, E.: Hemagglutination by measles virus. III. Identification of two different hemagglutinins. Virology19, 147–157 (1963).

    PubMed  Google Scholar 

  6. Norrby, E.: Separation of measles virus components by equilibrium centrifugation in CsCl gradient. Arch. ges. Virusforsch.14, 306–318 (1964).

    PubMed  Google Scholar 

  7. Clarke, D. H., andJ. Casals: Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Amer. J. trop. Med.7, 561–573 (1958).

    PubMed  Google Scholar 

  8. Neurath, A. R., andF. Sokol: Subunits of myxoviruses. IV. Haemolysin of para-influenza 1 (Sendai) virus. Acta Virol.6, 66–75 (1962).

    Google Scholar 

  9. Wako, H., R. Kawana, M. Kaneko, M. Matumoto, M. Mutai, Y. Saburi, andM. Nakamura: Clinical and antigenic effects in children of measles virus adapted to bovine kidney cell culture. Jap. J. exp. Med.31, 481–485 (1961).

    PubMed  Google Scholar 

  10. Mutai, M.: Isolation and identification of measles virus. Jap. J. exp. Med.29, 283–295 (1959).

    Google Scholar 

  11. Kärber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. Path. Pharm.162, 480–498 (1931).

    Google Scholar 

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Authors and Affiliations

  1. The Institute for Infectious Diseases, The University of Tokyo, Minatoku, Tokyo, Japan

    Minoru Matumoto

Authors
  1. Yasuo Saburi
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  2. Minoru Matumoto
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Saburi, Y., Matumoto, M. Assay of measles virus hemolysin and its antibody. Archiv f Virusforschung 17, 29–41 (1965). https://doi.org/10.1007/BF01242598

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  • DOI: https://doi.org/10.1007/BF01242598

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