Summary
Chemical analysis was performed on the highly purified Newcastle disease virus (NDV) obtained from infected allantoic fluid. Mean values of RNA content of the virus based on optical density at 260 mμ, phosphorus content and orcinol reaction which were carried out after the fractionation bySchmidt-Thannhauser-Schneider procedure modified byFleck andMunro were 0.72%, 0.99% and 0.92%, respectively. Contents of protein and lipid in the virus were also determined.
Isolation of the RNA from NDV was performed by hot-SDS-phenol extraction from32P-labeled virus and sedimentation patterns of the RNA in sucrose density gradients were examined. The sedimentation constant of NDV-RNA was found to be 55S, and this component was completely digested with ribonuclease. By this observation and the base composition previously determined, the RNA of NDV must be single stranded. The molecular weight of the RNA was estimated 5.8×106 at least from the weight and RNA content of the virus particle.
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Nakajima, H., Obara, J. Physicochemical studies of Newcastle disease virus. Archiv f Virusforschung 20, 287–295 (1967). https://doi.org/10.1007/BF01241948
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DOI: https://doi.org/10.1007/BF01241948