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In vitro secondary 19 S and 7 S antibody responses to poliovirus in membrane cultures of separated spleen cells

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Summary

Membrane cultures of separated spleen cells, from rabbits injected once with, poliovirus, formed antibodyin vitro for 2–3 weeks. Restimulation of spleen cells in suspension with homologous antigen evoked secondary antibody responses. The secondary 19 S antibody response was rapid and transient. The secondary 7 S response, which appeared to be biphasic, was of longer duration and reached peak titers about 12–13 days after antigenic restimulation. Antibody production was prolonged when spleen cells were cultured together with a feeder layer of rabbit embryo fibroblasts. Incorporation into the medium of chloramphenicol (50μ/ml.) or actinomycin D (5μ/ml.) inhibited the continued primary 19 S antibody synthesis.

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Author notes

  1. Sven -Eric Svehag (Fellow of The Helen Hay Whitney Foundation)

    Present address: Department of Virus Research, Karolinska Institutet Medical School, Stockholm, Sweden

Authors and Affiliations

  1. Department of Virology, The Public Health Research Institute of The City of New York, Inc., New York 9, New York

    Sven -Eric Svehag (Fellow of The Helen Hay Whitney Foundation)

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  1. Sven -Eric Svehag
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Additional information

This investigation was supported in part by research grant E-4360 from the National Institute of Allergy and Infectious Diseases, United States Public Health Service.

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Svehag, S.E. In vitro secondary 19 S and 7 S antibody responses to poliovirus in membrane cultures of separated spleen cells. Archiv f Virusforschung 15, 261–274 (1965). https://doi.org/10.1007/BF01241758

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  • DOI: https://doi.org/10.1007/BF01241758

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