Summary
Isolation and propagation of bluetongue virus was attempted in 4 tissue culture systems. Good results were demonstrated in all 4 systems using high titre virus well adapted to tissue culture. Using material containing early passage virus of low titre no isolations were achieved in LK cells maintained in medium containing calf serum; the results were poor in LK cells with horse serum in the medium and in BHK cells with calf serum in the medium and only in BHK cells with horse serum incorporated in the medium were satisfactory results obtained. Substances inhibitory to BTV were demonstrated in varying amounts in 5 different batches of calf sera.
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Pini, A., andW. Coackley: To be published.
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Pini, A., Coackley, W. & Ohder, H. The adverse effect of some calf sera on the isolation and propagation of bluetongue virus in tissue culture. Archiv f Virusforschung 18, 88–95 (1966). https://doi.org/10.1007/BF01241704
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DOI: https://doi.org/10.1007/BF01241704