Skip to main content
SpringerLink
Log in

Studies of the vaccine virus isolated during a trial with an attenuated poliovirus vaccine prepared in a human diploid cell strain, including use of a strain-specific bovine serum inhibitor

  • Published:
Archiv für die gesamte Virusforschung Aims and scope Submit manuscript

Summary

The characteristics of CHAT attenuated poliovirus grown in the WI-26 strain of human diploid fibroblasts were compared with the virus strains isolated from the feces of children who had received this vaccine.

Only type 1 strains were found. Only 1 out of 32 of these strains isolated 1–5 weeks after vaccination showed a partial increase in capacity to replicate at 40° (rct/40 intermediate). Eight of 158 fecal isolates showed some increase in replication at 39° (rct/39 intermediate).

Testing with strain-specific antiserum prepared by immunizing rabbits with CHAT virus disclosed loss of antigenic identity in isolates from 4 out of 5 children after 4 weeks but not after 1 week of intestinal multiplication of the vaccine.

A bovine serum that specifically inhibited plaque formation with the CHAT virus, yet did not affect any of 26 other type 1 strains — either attenuated or virulent — proved to be a genetically stable marker of the virus. Thirty-two of 33 fecal isolates were identified as the CHAT strain with this inhibitor.

Dextran sulfate did not inhibit plaque formation with the CHAT virus in contrast to its inhibitory effect on LSc2ab virus. The nonsensitive character of CHAT to dextran sulfate was unchanged in the 11 strains of CHAT virus derived by a single human passage of the vaccine.

The mean titer of CHAT virus measured in primary human tissue culture was log10 2.09 PFU less than the titer as measured in primary MKTC. Despite preparation of the vaccine in HDCS instead of in MKTC this property of CHAT, designated theH marker, was retained.

Our findings point to a high degree of genetic stability of the CHAT vaccine cultivated in HDCS.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pagano, J. S., M. Böttiger, J. O. Bonnevier, andS. Gard: Amer. J. Hyg.79, 74 (1964).

    Google Scholar 

  2. Ikić, D., B. Jancikić, M. Branica, andB. Mravunac: Symposium on the characterization and uses of human diploid cell strains, Opatija, Yugoslavia, 24–26 Sept. (1963).

  3. Buser, F., C. Fleury, M. DuPan, andE. Rohner: Schweiz. Med. Wschr., in press.

  4. Pagano, J. S., T. W. Norton, J. LaLiberté, andH. Koprowski: Unpublished.

  5. Wecker, E.: Virology10, 376 (1960).

    Google Scholar 

  6. Carp, R. I., andH. Koprowski: Virology16, 371 (1962).

    Google Scholar 

  7. Takemoto, K. K., andH. Kiebhauer: Virology17, 499 (1962).

    Google Scholar 

  8. Pagano, J. S., R. V. Gilden, andW. D. Sedwick: Fed. Proc.230, 508 (1964).

    Google Scholar 

  9. Hayflick, L., S. A. Plotkin, T. W. Norton, andH. Koprowski: Amer. J. Hyg.75, 240 (1962).

    Google Scholar 

  10. Gard, S.: Pan American Health Org., WHO, Sci. Publ. No. 50, 187 (1960).

    Google Scholar 

  11. Plotkin, S. A., B. J. Cohen, andH. Koprowski: Virology15, 473 (1961).

    Google Scholar 

  12. Pagano, J. S., R. I. Carp, J. LaLiberté, andD. Sedwick: Symposium on the characterization and uses of human diploid cell strains, Opatija, Yugoslavia, 24–26 Sept. (1963).

  13. Carp, R. I., andH. Kropowski: Virology16, 71 (1962).

    Google Scholar 

  14. Dane, D. S., G. W. A. Dick, M. Briggs, R. Nelson, J. McAlister, J. H. Connolly, F. McKeown, andC. M. B. Field: Brit. med. J.2, 269 (1961).

    Google Scholar 

  15. Dane, D. S., G. W. A. Dick, M. Briggs, R. Nelson, J. McAlister, J. H. Connolly, M. Haire, F. McKeown, andC. M. B. Field: Brit. med. J.2, 259 (1961).

    Google Scholar 

  16. Koprowski, H.: N. Y. Acad. Sci. (Spec. Publ.)5, 113 (1957).

    Google Scholar 

  17. Gard, S.: Exit poliomyelitis — what next? Virological and Epidemiological Contributions in Honor of John Rodman Paul. (Yale J. Biol. Med., Dec.–Feb. 1961/62.) 117, D. M. Horstmann, ed., Archon Books, Hamden, Conn. (1961).

    Google Scholar 

  18. Hayflick, L.: Symposium on the characterization and uses of human diploid cell strains, Opatija, Yugoslavia, 24–26 Sept. (1963).

  19. Habel, K.: Symposium on the characterization and uses of human diploid cell strains, Opatija, Yugoslavia, 24–26 Sept. (1963).

  20. Koprowski, H., R. Carp, T. W. Norton, B. Cohen, andS. Plotkin: Pan American Health Org., WHO, Sci. Publ. No. 50, 3 (1960).

    Google Scholar 

  21. Bodian, D.: Bull. Johns Hopk. Hosp.112, 225 (1963).

    Google Scholar 

  22. Wasserman, F. E., andJ. P. Fox: Arch. Path.74, 275 (1962).

    Google Scholar 

  23. Nakano, J. H., H. Gelfand, andJ. T. Cole: Amer. J. Hyg.78, 214 (1963).

    Google Scholar 

  24. Boué, A.: Unpublished investigations.

  25. Klein, M.: Ann. N. Y. Acad. Sci.70, 362 (1958).

    Google Scholar 

  26. Takemori, N., S. Nomura, M. Nakano, Y. Morioka, M. Henmi, andM. Kitaoka: Virology5, 30 (1958).

    Google Scholar 

  27. Takemoto, K. K., andK. Habel: Virology9, 228 (1959).

    Google Scholar 

  28. Plummer, G.: Nature197, 870 (1963).

    Google Scholar 

  29. Pagano, J. S.: Immunization of Infants with Attenuated Poliovirus Vaccines Prepared in Cultures of Human Diploid Cell Strains. Unpublished.

  30. Ledinko, N.: Virology20, 107 (1963).

    Google Scholar 

  31. Pagano, J. S.: Unpublished data.

Download references

Author information

Authors and Affiliations

  1. The Wistar Institute, Philadelphia, Pennsylvania

    Joseph S. Pagano M. D. & Margareta Böttiger M. D.

  2. The National Bacteriological Laboratory, Stockholm, Sweden

    Joseph S. Pagano M. D. & Margareta Böttiger M. D.

  3. Virus Diagnostic Section, National Bacteriological Laboratory, Stockholm

    Margareta Böttiger M. D.

Authors
  1. Joseph S. Pagano M. D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Margareta Böttiger M. D.
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Presented, in part, before the American Society of Immunologists, April, 1963.

Associate Member of the Wistar Institute and Associate in Medicine, The University of Pennsylvania, School of Medicine.

Supported by grant AI-01799-06 from the National Institute of Allergy and Infectious Diseases, United States Public Health Service.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pagano, J.S., Böttiger, M. Studies of the vaccine virus isolated during a trial with an attenuated poliovirus vaccine prepared in a human diploid cell strain, including use of a strain-specific bovine serum inhibitor. Archiv f Virusforschung 15, 19–34 (1964). https://doi.org/10.1007/BF01241418

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01241418

Keywords

Search

Navigation