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The soluble hemagglutinins of adenoviruses belonging to Rosen's subgroup III

II. The slowly sedimenting hemagglutinin

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Summary

Zonal centrifugation of soluble components of adenovirus types 1, 2, 5 and 6 revealed five different components that represented, listed in sequence of decreasing sedimentation rate, penton aggregates (rapidly sedimenting complete HA; RHA), hexons, isolated pentons, slowly sedimenting complete HA (SHA) and fibers.

SHA eluted immediately after fibers and distinctly before pentons in anion exchange chromatography. It differed from penton-associated complete HA by the facts that it (a) did not carry any toxin activity, (b) was resistant to trypsin treatment, (c) was unaffected by prolonged heating and incubation with a moderate concentration of guanidin-HCl and (d) did not increase in HA activity in the presence of heterotypic antisera.

A comparison of the behaviour of SHA in zonal centrifugation and in exclusion chromatography suggested it to be a markedly elongated structure. It is assumed to represent an aggregate of two fibers. This together with the observations on penton and capsid associated HA of adenoviruses belonging to subgroup III indicates that possession of at least two erythrocyte reactive sites is a prerequisite for any of the structures to exert hemagglutinating activity.

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References

  • Bauer, H., andR. Wigand: Eigenschaften der Adenovirus-Hämagglutinine. Z. Hyg. Infekt.-Kr.149, 96 (1963).

    Google Scholar 

  • Gelderblom, H., H. Bauer, H. Frank, andR. Wigand: The structure of group II adenoviruses. J. gen. Virol.1, 553 (1967).

    PubMed  Google Scholar 

  • Ginsberg, H. S., H. G. Pereira, R. C. Valentine, andW. C. Wilcox: A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology28, 782 (1966).

    PubMed  Google Scholar 

  • Huebner, R. J., H. G. Pereira, A. C. Allison, A. C. Hollinshead, andH. C. Turner: Production of type-specific C antigen in virus-free hamster tumor cells induced by adenovirus type 12. Proc. nat. Acad. Sci. (Wash.)51, 432 (1964).

    Google Scholar 

  • Kasel, J. A., W. P. Rowe, andJ. L. Nemes: Modification of erythrocyte receptors by a factor in adenovirus suspensions. Virology10, 388 (1960).

    PubMed  Google Scholar 

  • Norrby, E.: The relationship between the soluble antigens and the virion of adenovirus type 3. I. Morphological characteristics. Virology28, 236 (1966).

    PubMed  Google Scholar 

  • Norrby, E.: Comparison of soluble components of adenovirus types 3 and 11. J. gen. Virol.2, 135 (1968).

    PubMed  Google Scholar 

  • Norrby, E., M. Grönberg, andP. Magnusson: Virus-specific products appearing during the multiplication of adenovirus type 3. Unio Ncrdica Contra Cancrum. Symposium on Virus and Cancer p. 118 (1964).

  • Norrby, E., B. Nygren, P. Skaaret, andA. Lengyel: Separation and characterization of soluble adenovirus type 9 components. J. gen. Virol.1, 1101 (1967).

    Google Scholar 

  • Pereira, H. G.: Antigenic structure of non-infectious adenovirus materials. Nature (Lond.)186, 571 (1960).

    Google Scholar 

  • Pereira, H. G., and.M. V. T. de Figueiredo: Mechanism of hemagglutination by adenovirus types 1, 2, 4, 5 and 6. Virology18, 1 (1962).

    PubMed  Google Scholar 

  • Schmidt, N. J., C. J. King, andE. H. Lennette: Hemagglutination and hemagglutination-inhibition with adenovirus type 12. Proc. Soc. exp. Biol. (N.Y.)118, 208 (1965).

    Google Scholar 

  • Siegel, L. M., andK. J. Monty: Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductase. Biochim. biophys. Acta (Amst.)112, 346 (1966).

    Google Scholar 

  • Sober, H. A., andE. A. Peterson: Protein chromatography on anion exchange cellulose. Fed. Proc.17, 1116 (1958).

    PubMed  Google Scholar 

  • Wadell, G., E. Norrby, andU. Schönning: Ultrastructure of soluble antigens and the virion of adenovirus type 4. Arch. ges. Virusforsch.21, 234 (1967).

    PubMed  Google Scholar 

  • Wadell, G., andP. Skaaret: Separation of adenovirus type 4 components. Arch. ges. Virusforsch.21, 366 (1967).

    PubMed  Google Scholar 

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Author notes

  1. G. Wadell

    Present address: Department of Virology, Karolinska Institutet, Faculty of Medicine, University, Stockholm, Sweden

Authors and Affiliations

  1. Department of Virology, Karolinska Institutet, Faculty of Medicine, University, Stockholm, Sweden

    E. Norrby

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  1. G. Wadell
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  2. E. Norrby
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Supported by grants from the Swedish Medical Research Council (Project no. 16X-548-03).

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Wadell, G., Norrby, E. The soluble hemagglutinins of adenoviruses belonging to Rosen's subgroup III. Archiv f Virusforschung 26, 53–62 (1969). https://doi.org/10.1007/BF01241175

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  • DOI: https://doi.org/10.1007/BF01241175

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