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Transformation of sheep cells by simian virus 40

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Summary

Cells were cultured from seven separate foetuses of sheep and transformation assays using simian virus 40 were carried out as both liquid and agar assays. Consistent differences were found in the transformation frequencies observed in the different tissues cultured. Consistent differences were also observed between liquid and agar assays.

Batches of SV 40 virus prepared from the original stock showed a high particle count in electron microscopy but a relatively low infectious titre.

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References

  1. Aaronson, S. A.: Susceptibility of human cell strains to transformation by simian virus 40 and simian virus 40 deoxyribonucleic acid. J. Virol.6, 470–475 (1970).

    Google Scholar 

  2. Black, P. H., andW. P. Rowe: Transformation in hamster kidney monolayers by vacuolating virus, SV 40. Virology19, 107–109 (1963).

    Google Scholar 

  3. Black, P. H., andW. P. Rowe: An analysis of SV 40 induced transformation of hamster kidney tissuein vitro. I. General characteristics. Proc. nat. Acad. Sci. (Wash.)50, 606–613 (1963).

    Google Scholar 

  4. Black, P. H., andW. P. Rowe: SV 40 induced proliferation of tissue culture cells of rabbit, mouse and porcine origin. Proc. Soc. exp. Biol. (N.Y.)114, 721–727 (1963).

    Google Scholar 

  5. Bruere, A. N., andJoan Macnab: A cytogenetical investigation of six intersex sheep, shown to be freemartins. Res. vet. Sci.9, 170–180 (1968).

    Google Scholar 

  6. Diderholm, H., R. Berg, andT. Wesslen: Transformation of rat and guinea pig cellsin vitro by SV 40, and the transplantability of the transformed cells. International J. Cancer1, 139–146 (1966).

    Google Scholar 

  7. Diderholm, H., B. Stenvist, J. Ponten, andT. Wesslen: Transformation of bovine cellsin vitro after inoculation of simian virus 40 or JTS nucleic acid. Exp. Cell Res.37, 452–459 (1965).

    Google Scholar 

  8. Fernandes, M., and P. S.Moorhead: Transformation of African green monkey Kidney cultures infected with simian vacuolating virus (SV 40). Tex. Rep. Biol. Med.23 (1965).

  9. Jensen, F., H. Koprowski, andJ. A. Ponten: Rapid transformation of human fibroblast cultures by simian virus 40. Proc. nat. Acad. Sci. (Wash.)50, 343–348 (1963).

    Google Scholar 

  10. McPherson, I., andL. Montagnier: Agar suspension culture for the selective assay of cells transformed by Polyoma virus. Virology23, 291–294 (1964).

    Google Scholar 

  11. Mukergee, D., J. Bowen, andD. E. Anderson: Simian Papova virus 40 transformation of cells from cancer patient with XY/XXY mosaic Klinefelter's syndrome. Cancer Res.30, 1769–1772 (1970).

    Google Scholar 

  12. Rabson, A. S., R. L. Kirschstein, andF. Y. Legallais: Autologous implantation of Rhesus monkey cells “transformed”in vitro by Simian Virus 40. J. nat. Cancer Inst.35, 981–986 (1965).

    Google Scholar 

  13. Todaro, G. J., H. Green, andM. R. Swift: Susceptibility of human diploid fibroblasts strains to transformation by SV 40 virus. Science153, 1252–1254 (1966).

    Google Scholar 

  14. Todaro, G. J., andG. M. Martin: Increased susceptibility of Down's syndrome fibroblasts to transformation by SV 40. Proc. exp. Biol. (N.Y.)124, 1232–1236 (1967).

    Google Scholar 

  15. Uchida, S., andS. Watanabe: Tumorigenicity of the antigen forming defective virions of simian virus 40. Virology35, 166–169 (1968).

    Google Scholar 

  16. Uchida, S., andS. Watanabe: Transformation of Mouse 3 T 3 cells by T-antigen forming defective SV 40 virions (T particles). Virology39, 721–728 (1969).

    Google Scholar 

  17. Uchida, S., K. Yoshiike, S. Watanabe, andA. Furono: Antigenforming defective viruses of simian virus 40. Virology34, 1–8 (1968).

    Google Scholar 

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Macnab, J.C.M. Transformation of sheep cells by simian virus 40. Archiv f Virusforschung 37, 71–77 (1972). https://doi.org/10.1007/BF01241152

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  • DOI: https://doi.org/10.1007/BF01241152

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