Abstract
The influence of lindane (gamma-hexachlorocyclohexane) on fluidity of plasma membranes from rat renal cortical tubules has been investigated. Preincubation with lindane increased membrane fluidity. This effect was accompanied by (i) a decrease in the transport of glucose with regard to the controls and (ii) an inhibition of the β-adrenergic stimulatory activity upon cyclic AMP accumulation. However, a significant decrease of the membrane fluidity was found when rats were injected with lindane for 12 days. The injection of lindane exerted the opposite effect on the membrane proteins, the glucose transporter and the β-adrenergic receptor, enhancing the glucose uptake and increasing the isoproterenol-stimulated cycle AMP accumulation. A possible explanation of the difference could involve a resistance to membrane disordering by lindane through a regulatory mechanism that would balance the activity of many lindane-sensitive proteins in insecticide-injected rats.
Similar content being viewed by others
References
Lange, M., Nitzsche, K. and Zesch, A. (1981)Arch. Dermatol. Res. 271:387–399.
Davies, J. E., Dedhia, H. V., Morgade, A., Barquet, A. and Maibach, H. I. (1983)Arch. Dermatol. 119:142–144.
Zhu, J., Feng, Z. and Chem, J. (1986)Pest. Biochem. Physiol. 25:414–419.
Lopez-Aparicio, P., del Hoyo, N. and Pérez-Albarsanz, M. A. (1988)Pest. Biochem. Physiol. 31:109–119.
Engst, R., Macholz, R. M. and Kujawa, M. (1977)Residue Rev. 68:59–90.
Copeland, M. F., Chadwich, R. W., Cooke, N., Whitehouse, D. A. and Hill, D. M. (1986)J. Toxicol. Environ. Health 18:527–542.
Antunes-Maderia, M. C. and Madeira, V. M. C. (1985)Biochim. Biphys. Acta 820:165–172.
Hulth, L., Larsson, R., Carlsson, R. and Kihlstrom, J. E. (1976)Bull. Environ. Contam. Toxicol. 16:133–137.
Joy, R. M. (1982)Neurobehav. Toxicol. Teratol. 4:813–823.
Dikshith, T. S. S., Tandon, S. K., Datta, K. K., Gupta, P. K. and Behari, J. R. (1978)Environ. Res. 17:1–9.
Senar, S., Gutierrez-Ocaña, M. T., Pérez-Albarsanz, M. A. and Recio, M. N. (1991)Biosci. Rep. 11:101–109.
Pérez-Albarsanz, M. A., López-Aparicio, P., Senar, S. and Recio, M. N. (1991)Biochim. Biophys. Acta 1066:124–130.
Bondy, S. C. and Halsall, L. (1988)Neurotoxicol. 9:645–652.
Kaplan, S. S., Zdziarski, U. E., Kuhns, D. B. and Basford, R. E. (1988)Blood 71:677–683.
Joy, R. M. and Burns, V. W. (1988)Neurotoxicol. 9:637–644.
Crouch, M. F. and Roberts, M. L. (1985)Biochim. Biphys. Acta 844:149–157.
Parries, G. S. and Hokin-Neaverson, M. (1985)J. Biol. Chem. 260:2687–2693.
Pulido, J. A., del Hoyo, N. and Pérez-Albarsanz, M. A. (1992)Life Sci. 50:1585–1596.
del Hoyo, N., Pulido, J. A. and Pérez-Albarsanz, M. A. (1993)Biosci. Rep. 13:119–126.
Ogata, N., Vogel, S. M. and Narahashi, T. (1988)FASEB J. 2:2895–2900.
Llorens, J., Suñol, C., Tusell, J. M. and Rodriguez-Farre, E. (1990)Neurotoxicol. Teratol. 12:607–610.
Wafford, K. A., Sattelle, D. B., Gant, D. B., Eldefrawi, A. T. and Eldefrawi, M. E. (1989)Pest. Biochem. Physiol 33:213–219.
Carrero, I., Fernández-Moreno, M. D., Pérez-Albarsanz, M. A. and Prieto, J. C. (1989)Biochem. Biophys. Res. Commun. 159:1391–1396.
Carrero, I., Pérez-Albarsanz, M. A., Carmena, M. J. and Prieto, J. C. (1990)Pest. Biochem. Physiol. 38:197–203.
López-Aparicio, P., Recio, M. N., Prieto, J. C., Carmena, M. J. and Pérez-Albarsanz, M. A. (1991)Life Sci. 49:1141–1154.
Carrero, I., Pérez-Albarsanz, M. A., Recio, M. N. and Prieto, J. C. (1992)Toxic, in Vitro 6:7–9.
Carrero, I., Rodriguez-Henche, N., Guijarro, L. G., Recio, M. N., Pérez-Albarsanz, M. A. and Prieto, J. C. (1993)Cell. Signal. 5:453–462.
Pulido, J. A. del Hoyo, N. and Pérez-Albarsanz, M. A. (1990)Life Sci. 47:1009–1107.
Gutiérrez-Ocaña, M. T., Senar, S., Pérez-Albarsanz, M. A. and Recio, M. N. (1992)Biosci. Rep. 12:303–311.
Vinay, P., Gougoux, A. and Lemieux, G. (1981)Am. J. Physiol. 241:F403-F411.
Lowry, O. H., Rosebrough, N. J., Farr, A. and Randall, R. J. (1951)J. Biol. Chem. 193:265–275.
Carmena, M. J. and Prieto, J. C. (1985)Biosci. Rep. 5:791–797.
Gilman, A. G. (1970)Proc. Natl. Acad. Sci. USA 67:305–312.
Sakhrani, L. M., Badie-Dezfooly, B., Trizna, W., Mikhail, N., Lowe, A. G., Taub, M. and Fine, L. G. (1984)Am. J. Physiol. 246:757–764.
Antunes-Madeira, M. C. and Madeira, V. M. C. (1989)Biochim. Biophys. Acta 982:161–166.
Antunes-Madeira, M. C. and Madeira, V. M. C. (1990)Biochim. Biophys. Acta 1023:469–474.
Carrero, I., Prieto, J. C., Recio, M. N., del Hoyo, N. and Pérez-Albarsanz, M. A. (1993)Pest. Biochem. Physiol. 47:69–78.
Kyrklund, T., Kjellstrand, P. and Haglid, K. G. (1987)Toxicology 45:123–133.
Friedlander, G., Shahedi, M., Le Grimellec, C. and Amiel, C. (1988)J. Biol. Chem. 263:11183–11188.
Stubbs, C. D., Williams, B. W., Pryon, C. L. and Rubin, E. (1988)Arch. Biochem. Biophys. 262:560–573.
Hoek, J. B. and Taraschi, T. F. (1988)Trend Biochem. Sci. 13:269–274.
Camacho, J. and Rubalcava, B. (1984)Biochem. Biophys. Acta 766:97–104.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
López-Aparicio, P., Recio, M.N., Prieto, J.C. et al. Role of lindane in membranes. Effects on membrane fluidity and activity of membrane-bound proteins. Biosci Rep 14, 131–138 (1994). https://doi.org/10.1007/BF01240245
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01240245