Summary
Atomic absorption spectroscopy has been used to determine the copper content of commercial chlorophyllins and of the iron in Cytochrome-C. The procedure is rapid and direct as preparation involves only weighing and dissolution of the sample. The precision of the methods is excellent. The values obtained compare favorably with those obtained by the standard colorimetric methods.
Zusammenfassung
Mit Hilfe der Atomabsorption wurde der Kupfergehalt in käuflichem Chlorophyllin und der Eisengehalt in Cytochrom C bestimmt. Da zur Vorbereitung nur die Einwaage und die Auflösung der Probe nötig sind, führt das Verfahren rasch ans Ziel. Seine Genauigkeit ist ausgezeichnet und seine Resultate entsprechen den Ergebnissen der kolorimetrischen Standardmethoden.
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References
P. G. Stecher (Ed.), The Merck Index, 9th edition. Rahway: Merck and Co. 1968. p. 245.
H. H. Strain andW. A. Svec, in:L. P. Vernon andG. R. Seely (Ed.), The Chlorophylls. New York: Academic Press. 1966. pp. 21–66.
Anon. Fed. Regist.34 (80), 6975 (1969).
J. C. Klephart, Economic Botany9, 3 (1955).
E. Margoliash, Ann. Rev. Biochem.30, 549 (1960).
J. F. Morrison, Ann. Rev. Biochem.30, 11 (1960).
W. Jankowski andZ. Ziemski, Otolaryngol. Pol.22 (4), 513 (1968).
B. M. Blank andK. Morgareidge, J. Amer. Pharm. Assoc.46, 73 (1957).
W. L. Wuggatzer andJ. E. Christian, J. Amer. Pharm. Assoc.44, 645 (1955).
J. H. C. Smith andC. S. French, Ann. Rev. Plant Physiol.14, 181 (1963).
H. Diehl andG. F. Smith, The Copper Reagents: Cuproine, Neocuproine, Bathocuproine. Columbus: G. Frederick Smith Chemical Co. 1958.
H. Diehl andG. F. Smith, The Iron Reagents: Bathophenanthroline, Bathophenanthroline-disulfonic acid, 2,4,6-Tripyridyl-s-triazine, Phenyl-2-pyridyl ketoxime. Columbus: G. Frederick Smith Chemical Co. 1965.
J. Pijck, J. Gillis, andJ. Hoste, Intern. J. Appl. Research and Isotopes10, 149 (1961).
J. Pijck, J. Gillis, andJ. Hoste, Proceedings of the International Symposium on Micro Chemistry. Birmingham, 1958, p. 48. London: Pergamon Press. 1958.
T. T. Gorsuch, Analyst84, 135 (1959).
Pharmacopoeia of The United States, 16th Revision. Easton: Mack Publishing Co. 1960.
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Toralballa, G.C., Spielholtz, G.I. & Steinberg, R.J. The determination of metal content in biochemical products by atomic absorption spectroscopy. I. Mikrochim Acta 58, 879–883 (1970). https://doi.org/10.1007/BF01225713
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DOI: https://doi.org/10.1007/BF01225713