Summary
A convenient method for measuring the release of gut glucagon-like immunoreactivity (G-LI) from rat jejunal pieces incubated ‘in vitro’ is described. The method utilises Trasylol, EDTA, and excessive glucagon antibody to prevent GLI breakdown. There was satisfactory recovery of added pancreatic glucagon to the incubates, and a highly significant release of GLI was recorded when high glucose concentrations (5–40 g%) were incubated with the pieces.
Résumé
Les auteurs décrivent une méthode commode pour mesurer la libération de la substance intestinale immunologiquement glucagon-like (GLI) à partir de fragments de jéjunum de rat, incubés “in vitro”. La méthode utilise du trasylol, de l'EDTA et un excès d'anticorps anti-glucagon pour empêcher la dégradation du GLI. Il y avait une récupération satisfaisante du glucagon pancréatique ajouté aux incubats, et une libération de GLI hautement significative a été enregistrée lorsque de fortes de concentrations de glucose (5– 40 g%) étaient mises à incuber avec les fragments.
Zusammenfassung
Es wird eine brauchbare Methode zur Bestimmung der Freisetzung von glucagonähnlicher immunologischer Aktivität (GLI) des Darmes beschrieben. Die Methode benutzt Trasylol, EDTA und einen Überschuß von Glucagonantikörpern, um den Abbau von GLI zu verhindern. Die Rückgewinnung von zugefügtem Pankreasglucagon aus den Inkubaten war zufriedenstellend und eine hoch signifikante Freisetzung von GLI wurde beobachtet, wenn hohe Glucosekonzentrationen (5–40%) mit den Darmstücken inkubiert wurden.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Buchanan, K.D., McCarroll, A.M.: Radio-immunoassay methods, p. 266. Edinburgh and London: Churchill Livingstone 1971.
Greenwood, F.C., Hunter, W.M., Glover, J.S.: The preparation of131I-labelled human growth hormone of high specific radioactivity. Biochem. J.89, 114–123 (1963).
Luyckx, A. S., Lefebvre, P.J.: Protein and polypeptide hormones, p. 884. International Congress Series No. 161. Amsterdam: Excerpta Medica Foundation 1968.
Malaisse, W., Malaisse-Lagae, F.: A new method for the measurement in vitro of pancreatic insulin secretion. Endocrinology80, 99–108 (1967).
Samols, E., Tyler, J., Magyesi, C., Marks, V.: Immunochemical glucagon in human pancreas, gut and plasma. Lancet1966 II, 727–729.
— —, Marri, G., Marks, V.: Stimulation of glucagon secretion by oral glucose. Lancet1965 II, 1257–1259.
Unger, H.H., Ketterer, M., Eisentraut, A.M.: Distribution of immunoassayable glucagon in gastrointestinal tissues. Metabolism15, 865–867 (1966).
Valverde, I., Rigopoulou, D., Marco, J., Faloona, G.R., Unger, R.H.: Characterisation of glucagon-like immunoreactivity (GLI). Diabetes19, 614–623 (1970a).
— — — — —: Molecular size of extractable glucagon and glucagon-like immunoreactivity (GLI) in plasma. Diabetes19, 624–629 (1970b).
Wilson, T.H., Wiseman, G.: The use of sacs of everted small intestine for the study of the transference of substances from the mucosal to the serosal surface. J. Physiol.123, 116–125 (1954).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Zandomeneghi, R., Buchanan, K.D. A method for measuring the release of gut glucagon-like immunoreactivity from rat jejunum „In Vitro“. Diabetologia 8, 283–286 (1972). https://doi.org/10.1007/BF01225572
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01225572