Summary
Insulin fixation and glucose uptake have been studied in the forearm tissues of nine lean non-diabetic men for three consecutive hours, during a fasting state, an intra-arterial infusion of a small dose of insulin (25–100 mU) and a recovery period. When insulin was administered, the arterial plasma immuno-reactive insulin rose to levels ranging from 15 to 65 μU/ml; the tissue insulin fixation was significantly increased during this period, but no effect on tissue glucose uptake was observed. A close correlation was found between the arterial plasma insulin concentration and the tissue insulin fixation. — The injection of 200 mg glucose intraarterially at the end of the study resulted in a reversal of the arterio-venous gradient of plasma insulin concentration. — The results show that large amounts of circulating insulin can be bound to the forearm tissues without exerting an effect on glucose uptake. It is suggested that the binding of insulin occurs at the vascular endothelium and that it is reversible by increased concentrations of blood glucose.
Résumé
La fixation d'insuline et la captation de glucose par les tissus de l'avant-bras ont été étudiées chez 9 sujets non diabétiques de poids normal, au cours de 3 périodes d'observation d'une heure chacune: à jeun, pendant la perfusion intra-artérielle de petites doses d'insuline (25–100 mU) et après l'arrêt de la perfusion. Pendant l'administration d'insuline, l'insuline plasmatique dans l'artère a atteint des taux variant de 15 à 65 μU/ml; simultanément, la fixation d'insuline aux tissus de l'avantbras s'est élevée de façon significative mais aucun effet sur la captation de glucose n'a été observé. Il existe une étroite corrélation entre les taux d'insuline plasmatique dans l'artère et la fixation d'insuline aux tissus. — Au terme des épreuves, 200 mg de glucose en solution saline ont été injectés par voi intra-artérielle dans l'avant-bras et ont provoqué l'inversion du gradient artério-veineux de concentration plasmatique d'insuline. — Les résultats montrent que des quantités appréciables d'insuline circulante peuvent être fixées par les tissus de l'avant-bras sans exercer d'effet sur la captation de glucose. Il est possible que l'insuline se lie à l'endothélium capillaire et qu'elle en soit libérée par un accroissement du taux de glucose sanguin.
Zusammenfassung
Am Vorderarmgewebe von 9 normalgewichtigen, nicht-diabetischen Versuchspersonen, wurde die Insulinfixation und die Glucoseaufnahme in 3 aufeinanderfolgenden Stunden gemessen: Während des nüchternen Zustandes, während einer intraarteriellen Infusion von kleinen Insulinmengen (25 bis 100 mE) und einer Erholungsphase. Wenn Insulin verabfolgt wurde, stieg der Plasmaspiegel des arteriellen immunoreaktiven Insulins auf Werte von 15 bis 65 μE/ml. Die Fixation des Insulins im Gewebe stieg während dieser Periode signifikant an; ein Effekt auf die Glucoseaufnahme des Gewebes wurde aber nicht beobachtet. Zwischen der Konzentration des arteriellen Plasmainsulins und der Fixation des Insulins im Gewebe ließ sich eine enge Korrelation feststellen. — Die Injektion von 200 mg Glucose intraarteriell am Ende der Untersuchung ergab eine Umkehr des arterio-venösen Gradienten der Insulinkonzentration im Plasma. — Die Ergebnisse zeigen, daß große Mengen von zirkulierendem Insulin an das Gewebe des Vorderarms gebunden werden können, ohne daß sie einen Effekt auf die Glucoseaufnahme ausüben. Man muß annehmen, daß die Insulinbindung am Gefäßendothel erfolgt und daß sie durch steigende Konzentration der Blutglucose reversibel ist.
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Rasio, E., Whichelow, M.J., Butterfield, W.J.H. et al. Insulin fixation and glucose uptake by forearm tissues in response to infusions of physiological amounts of insulin in non-diabetic subjects. Diabetologia 8, 244–249 (1972). https://doi.org/10.1007/BF01225567
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DOI: https://doi.org/10.1007/BF01225567