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Genetic polymorphism of the single locus probes pL159-1 and pL355-8

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Summary

The genetic polymorphism of the single-locus probes pL159-1 (D18S17) and pL355-8 (D20S15) was investigated in 445 unrelated individuals usingPstl as restriction enzyme. Fragment size calculations were obtained using the molecular weight size marker MW-SBH. The basic relationship between migration distance and molecular weight was transformed using an exponential function. Fragment size frequency data show 2 peaks for pL159-1 at 4.36 kb (2.36%) and 4.67 kb (6.29%) and one peak for pL355-8 at 6.04 kb (5.73%). The rate of heterozygosity exceeded 70% for both probes.

Zusammenfassung

Populationsgenetische Untersuchungen der Single-Locus-Sonden pL159-1 (D18S17) und pL355-8 (D20S15) wurden anhand von 445 unverwandten Testpersonen durchgeführt. Als Restriktionsenzym wurde Pst I verwendet. Die Bestimmung der Fragmentgrößen erfolgte mittels des Molecular Weight Size Markes MW-SBH. Die zugrunde liegende Beziehung zwischen Migrationsdistanz und Molekulargewicht wird am ehesten durch eine Exponentialfunktion beschrieben. Erkennbar wird in der statistischen Häufigkeitsverteilung bei der Sonde pL159-1 eine Zweigipfligkeit, die mit 2.36% bei 4.36 kb und mit 6.29% bei 4.67 kb lokalisiert ist, während die Sonde pL355-8 nur einen Peak bei 6.04 mit 5.73% aufweist. Die Heterozygotenrate beider Sonden liegt bei mehr als 70%.

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Authors and Affiliations

  1. Institut für Anthropologie und Humangenetik, Wilhelmstrasse 27, D-72074, Tübingen, Germany

    K. Pfaff, C. Luckenbach & H. Ritter

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  1. K. Pfaff
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  2. C. Luckenbach
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  3. H. Ritter
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Pfaff, K., Luckenbach, C. & Ritter, H. Genetic polymorphism of the single locus probes pL159-1 and pL355-8. Int J Leg Med 106, 163–167 (1993). https://doi.org/10.1007/BF01225241

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  • DOI: https://doi.org/10.1007/BF01225241

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