Summary
Glucose oxidation and lipogenesis were studied in isolated adipocytes from control and nonobese, experimentally hyperinsulinaemic rats. In cells from the hyperinsulinaemic animals oxidation of either [1-14C]- or [6-14C] glucose was increased in the presence or absence of insulin, at substrate concentrations from 0.1 to 20 mmol/l. Glucose incorporation into total triglycerides and fatty acids was also increased. These enhanced rates of glucose metabolism were due to increased activity of the glucose transport system in addition to increased activity of intracellular glucose metabolism. Therefore, these data indicate that insulin can influence long term glucose homoeostasis by augmenting the overall cellular capacity for glucose metabolism at several loci.
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Dr. Kobayashi was an NIH Postdoctoral Trainee during the course of this work supported by NIH NIAMDD Training grant #AM 07217
Dr. Olefsky was a Clinical Investigator with the Veterans Administration during the course of this work
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Kobayashi, M., Olefsky, J.M. Effect of experimental hyperinsulinaemia on intracellular glucose metabolism of isolated adipocytes. Diabetologia 17, 111–116 (1979). https://doi.org/10.1007/BF01222211
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DOI: https://doi.org/10.1007/BF01222211