Summary
Population studies on Caucasians from northwest Germany were carried out using the short tandem repeat (STR) systems SE 33 (Locus: ACTBP2) and TC 11 (Locus: 11p15.5). After electrophoresis in PAG 26 alleles could be identified for SE 33 in a sample size of 180 unrelated individuals and 6 alleles were found for TC 11 in 110 individuals. The combined mean exclusion chance for both systems was 0.96 and the discrimination index 0.999. No significant deviations from Hardy-Weinberg equilibrium could be demonstrated. In a small sample of families (SE 33 −n = 21; TC 11 −n = 30) no new mutations could be found. Positive and reproducible results for both STRs could be obtained from 50 pg template DNA.
Zusammenfassung
Populationsstichproben nordwestdeutscher Kaukasier wurden mit den beiden Short tandem repeat (STR)-Systemen SE 33 (Locus: ACTBP2) and TC11 (Locus: 11p15.5) untersucht. Nach elektrophoretischer Auftrennung in PAG konnten 26 Allele für SE 33 in einer Bevölkerungsstichprobe von 180 nicht verwandten Personen und 6 Allele für TC 11 in einer Stichprobe von 110 Individuen differenziert werden. Der resultierende kombinierte AVACH-Wert für beide Systeme lag bei 0.96, der entsprechende Diskriminationsindex bei 0.999. Eine signifikante Abweichung vom Hardy-Weinberg-Gleichgewicht wurde nicht festgestellt. Erste Familienstudien (SE 33 −n = 21; TC 11 −n = 30) gaben keinen Hinweis auf Neumutation. Die Nachweissensitivität beider STR's lag im Bereich von 50 pg template DNA.
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Wiegand, P., Budowle, B., Rand, S. et al. Forensic validation of the STR systems SE 33 and TC 11. Int J Leg Med 105, 315–320 (1993). https://doi.org/10.1007/BF01222114
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DOI: https://doi.org/10.1007/BF01222114