Summary
In perifused pancreatic islets, the fluorescence of oxidized flavoproteins (FAD) was recorded continuously. Elevation of glucose concentration in the medium from 0 or 5 mM to 20 mM led to decrease in FAD-fluorescence beginning 10 sec after change of medium. L-leucine (10 mM), (±)-b-BCH (20 mM) andα-ketoisocaproic acid (10 mM) caused typical kinetics of FAD-fluorescence decrease. The results are interpreted to indicate rapid changes of the functional state of B-cell mitochondria induced by the above-mentioned stimulators of insulin release.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Panten, U., Christians, J., v. Kriegstein, E., Poser, W., Hasselblatt, A.: Effect of carbohydrates upon fluorescence of reduced pyridine nucleotides from perifused isolated pancreatic islets. Diabetologia9, 477–482 (1973)
Panten, U., v. Kriegstein, E., Poser, W., Schönborn, J., Hasselblatt, A.: Effect of L-leucine andα-ketoisocaproic acid upon insulin secretion and metabolism of isolated pancreatic islets. FEBS Letters20, 225–228 (1972)
Panten, U., Christians, J.: Effects of 2-endo-aminonorbornane-2-carboxylic acid upon insulin secretion and fluorescence of reduced pyridine nucleotides of isolated perifused pancreatic islets. Naunyn-Schmiedeberg's Arch. Pharmacol.276, 55–62 (1973)
Panten, U., Christians, J., v. Kriegstein, E., Poser, W., Hasselblatt, A.: Studies on the mechanism of L-leucine- andα-ketoisocaproic acid-induced insulin release from perifused isolated pancreatic islets. Diabetologia9, 149–154 (1974)
Panten, U.: Amino acids and insulin secretion. In: Handbuch der exp. Pharmakologie, Vol. 32/2, pp. 115–128. Berlin-Heidelberg-New York: Springer 1975
Chance, B., Mayer, D., Rossini, L.: A time-sharing instrument for direct readout of oxidation-reduction states in intra-cellular compartments of cardiac tissue. JEEE. Trans. Biomedical Eng.17, 118–121 (1970)
Chance, B., Salkovitz, J.A., Kovach, A.G.B.: Kinetics of mitochondrial flavoprotein and pyridine nucleotide in perfused heart. Amer. J. Physiol.223, 207–218 (1972)
Scholz, R., Thurman, R.G., Williamson, J.R., Chance, B., Bücher, Th.: Flavin and pyridine nucleotide oxidation-reduction changes in perfused rat liver. I. Anoxia and subcellular localization of fluorescent flavoproteins. J. biol. Chem.244, 2317–2324 (1969)
Panten, U., Ishida, H.: Flavin fluorescence of isolated pancreatic islets. Naunyn-Schmiedeberg's Arch. Pharmacol. Suppl.282, R 73 (1974)
Tager, H.S., Christensen, H.N.: 2-Aminonorbornane-2-carboxylic acid. Preparation, properties and identification of the four isomers. J. Amer. chem. Soc.94, 968–972 (1972)
Umbreit, W.W.: Methods for preparation and study of tissue. In: Manometric techniques ed. W.W. Umbreit, R.H. Burris, J.F. Stauffer, p. 135. Minneapolis: Burgess Publ. Co. 1957
Hellerström, C.: A method for the microdissection of intact pancreatic islets of mammals. Acta endocr. (Kbh.)45, 122–132 (1964)
Panten, U., dal Ri, H., Poser, W., Hasselblatt, A.: Eine Methode der Gewebsumströmung für Fluoreszenzmessungen. Pflügers Arch. ges. Physiol.323, 86–90 (1971)
Panten, U.: Untersuchungen über Stoffwechsel und Insulin-sekretion isolierter Pankreasinseln. Habilitationsschrift Universität Göttingen 1971
Krebs, H.A., Henseleit, K.: Untersuchungen über die Harnstoffbildung im Tierkörper. Hoppe-Seylers Z. physiol. Chem.210, 33 (1932)
Zaharko, D.S., Beck, L.V.: Studies of a simplified plasma insulin immunoassay using cellulose powder. Diabetes17, 444–457 (1968)
Chapman, J.B.: Fluorometric studies of oxidative metabolism in isolated papillary muscle. J. gen. Physiol.59, 135–154 (1972)
Hellmann, B.: Studies in obese-hyperglycemic mice. Ann. N. Y. Acad. Sci.131, 541–558 (1965)
Hellerström, C.: Effects of carbohydrates on the oxygen consumption of isolated pancreatic islets of mice. Endocrinology81, 105–112 (1967)
Aubert, X., Chance, B., Keynes, R.D.: Optical studies of biochemical events in the electric organ of Electrophorus. Proc. roy. Soc,B 160, 211–245 (1964)
Brauser, B., Bücher, Th., Dolivo, M.: Redox transitions of cytochromes and pyridine nucleotides upon stimulation of an isolated ganglion. FEBS Letters8, 297–300 (1970)
Landowne, D., Ritchie, J.M.: On the control of glycogenolysis in mammalian nervous tissue by calcium. J. Physiol.212, 503–517 (1971)
Peng, C.F., Price, D.W., Bhuvaneswaran, C., Wadkins, C.L.: Factors that influence phosphoenolpyruvate-induced calcium efflux from rat liver mitochondria. Biochem. biophys. Res. Commun.56, 134–141 (1974)
Patriarca, P.L., Carafoli, E.: A study of the intracellular transport of calcium in rat heart. J. cell. comp. Physiol.72, 29–38 (1968)
Carafoli, E., Dabrowska, R., Covetti, F., Tiozzo, R., Drabikowski, W.: Anin vitro study of the interaction of heart mitochondria with troponin-bound Ca2+. Biochem. biophys. Res. Commun.62, 908–912 (1975)
Douglas, W.W.: Stimulus-secretion coupling: The concept and clues from chromaffin and other cells. Brit. J. Pharmacol.34, 451–474 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Panten, U., Ishida, H. Fluorescence of oxidized flavoproteins from perifused isolated pancreatic islets. Diabetologia 11, 569–573 (1975). https://doi.org/10.1007/BF01222108
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01222108