Summary
The effect of serotonin on insulin secretion and biosynthesis was studied using isolated islets of mice. Serotonin produced a small stimulatory effect on insulin secretion when glucose was present in the incubation medium at a low concentration. On the other hand, an inhibition of insulin secretion was obtained with serotonin when glucose in the medium reached 3.0 mg/ml concentration. No significant effect of serotonin was obtained on insulin biosynthesis, neither in the presence of low nor with a high glucose concentration. These results suggest that the effect of this monoamine on insulin secretion is not mediated via its effect on insulin biosynthesis.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Cegrell, L., Falck, B., Hellman, B.: Monoaminergic mechanisms in the endocrine pancreas. In: The structure and metabolism of the pancreatic islets, S.E. Brolin, B. Hellman, and H. Knutson, (eds.) p. 426–435. New York: MacMillan 1964
Cegrell, L.: The occurrence of biogenic monoamines in the mammalian endocrine pancreas. Acta physiol. scand.314, (Suppl.) 1–60 (1968)
Falck, B., Hellman, B.: A fluorescent reaction for monoamines in the insulin producing cells of guinea pig. Acta endocr. (Kbh.)45, 133–138 (1964)
Feldman, J.M., Lebovitz, H.E.: Serotonin inhibition of in vitro insulin release from golden hamster pancreas. Endocrinology86, 66–70 (1970a)
Feldman, J.M., Lebovitz, H.E.: Specifity of serotonin inhibition of insulin release from golden hamster pancreas. Diabetes19, 475–479 (1970b)
Feldman, J.M., Lebovitz, H.E.: Mechanism of epinephrine and serotonin inhibition of insulin release in the golden hamsterin vitro. Diabetes19, 480–486 (1970c)
Gagliardino, J.J., Hernández, R.E., Rodriguez, R.R., Lauri, H.C.: Stimulatory effect of nialamide on serum levels of insulin. Amer. J. Physiol.219, 314–317 (1970)
Gagliardino, J.J., Iturriza, F.C., Hernández, R.E., Zieher, L.M.: Effect of catecholamine precursors on insulin secretion. Endocrinology87, 823–825 (1970)
Gagliardino, J.J., Zieher, L.M., Iturriza, F.C., Hernández, R.E., Rodriguez, R.R.: Insulin release and glucose changes induced by serotonin. Horm. Metab. Res.3, 145–150 (1971)
Hakanson, R., Lundquist, I., Rerup, C.: On the hyperglycemic effect of DOPA and dopamine. Europ. J. Pharmacol.1, 114–119 (1967)
Hernández, R.E., Gagliardino, J.J., Rodriguez, R.R.: In vitro insulinogenic effect of two monoamineoxidase inhibitors. Diabetes18, 417–420 (1969)
Jaim-Etcheverry, G., Zieher, L.M.: Electron microscopic cytochemistry of 5-hydroxytryptamine in the beta cell of guinea pig endocrine pancreas. Endocrinology83, 917–923 (1968)
Lacy, P., Kostianovsky, M.: Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes16, 35–39 (1967)
Lernmark, A.: The significance of 5-hydroxytryptamine for insulin secretion in the mouse. Horm. Metab. Res.3, 305–309 (1971)
Lundquist, I., Ekholm, R., Ericson, L.E.: Monoamines in the pancreatic islets of the mouse. 5-hydroxytryptamine as an intracellular modifier of insulin secretion and the hypoglycemic effect of] monoamine oxidase inhibitors. Diabetologia7, 414–422 (1971)
Lundquist, I.: Insulin secretion: its regulation by monoamines and acid amyloglucosidase. Acta physiol. scand.372, (Suppl.) 1–47 (1971)
Maier, V., Pfeiffer, E.F.: Biosynthese und Sekretion von Insulin nach Behandlung von Langerhansschen Inseln mit Neuraminidase. Hoppe-Seyler's Z. Physiol. Chem.353, 1546–1547 (1972)
Melani, F., Ditschuneit, H., Bartelt, K., Friedrich, H., Pfeiffer, E.F.: Über die radioimmunologische Bestimmung von Insulin im Blut. Klin. Wschr.43, 1000–1007 (1965)
Pfeiffer, E.F., Raptis, S., Fußgänger, R.: Gastrointestinal hormones and islet function. In: Handbook of Experimental Pharmacology. Heffter-Heubner, New Series, Vol. 34, E. Jores and V. Mutt (eds.), p. 259–310. Berlin, Heidelberg, New York: Springer Verlag 1973
Schatz, H., Maier, V., Hinz, M., Nierle, C., Pfeiffer, E.F.: Stimulation of3H-leucine incorporation into the proinsulin and insulin fraction of isolated pancreatic mouse islets in the presence of glucagon, theophylline and cyclic AMP. Diabetes22, 433–441 (1973)
Tamarit-Rodriguez, J., Goberna, R., Tamarit, J.: Effect of glucose-1-phosphate on insulin release in the isolated perfused rat pancreas. Diabetologia9, 92 (1973)
Telib, M., Raptis, S., Schröder, K.E., Pfeiffer, E.F.: Serotonin and insulin release in vitro. Diabetologia4, 253–256 (1968)
Author information
Authors and Affiliations
Additional information
Supported by Deutsche Forschungsgemeinschaft Bonn-Bad Godesberg, SFB 87 Ulm
Rights and permissions
About this article
Cite this article
Gagliardino, J.J., Nierle, C. & Pfeiffer, E.F. The effect of serotonin on in vitro insulin secretion and biosynthesis in mice. Diabetologia 10, 411–414 (1974). https://doi.org/10.1007/BF01221630
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01221630