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TLC technique for the detection and determination of gamma- BHC in biological material

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Summary

Five isomers present in technical benzene hexachloride were successfully separated by TLC on silica-gel G impregnated with silver nitrate solution. The γ-isomer was identified by running a control sample of γ-BHC. Measurement of the spot area was found suitable for the estimation of 5–20μg ofγ-BHC, and was applied to analysis of autopsy tissues. The limit of identification is 0.1μg.

Zusammenfassung

Auf mit Silbernitratlösung imprägniertem Kieselgel G konnten dünn-schichtchromatographisch fünf Isomere aus technischem Hexachlorbenzol erfolgreich getrennt werden. Dasγ-Isomere wurde mit Hilfe einer Vergleichsprobe identifiziert. Die Planimetrie der Flecken eignet sich zur Schätzung von 5–20 μgγ-Hexachlorbenzol. Die Methode wurde zur Untersuchung von Autopsieproben angewendet. Die Nachweisgrenze beträgt 0,1μg.

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Authors and Affiliations

  1. Toxicology Division, Chemical Examiner's Laboratory, Agra, India

    S. N. Tewari & I. C. Sharma

Authors
  1. S. N. Tewari
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  2. I. C. Sharma
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Tewari, S.N., Sharma, I.C. TLC technique for the detection and determination of gamma- BHC in biological material. Mikrochim Acta 66, 323–326 (1976). https://doi.org/10.1007/BF01220275

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  • DOI: https://doi.org/10.1007/BF01220275

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