Summary
The immunization of mice with impure, once crystallized, porcine insulin in different doses over 3 months led to a high antibody titre with 1 μg monocomponent(-MC-)porcine insulin effected only a low, antibody response after 3 months of immunization, thus proving a low immunogenicity. A single insulin adjuvant immunization differentiated between stimulated, normally reacting and tolerant animals. In stimulated animals with high titre antibodies no insulitis was found, but some animals tolerant towards insulin produced an insulitis, suggesting that this cellular immune reaction may not be induced by true Sanger insulin. The antigen responsible for insulitis may be an antigenically independent antigen contained in crystallized insulin, or an antigenically related derivative of insulin.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Federlin, K., Renold, A.E., Pfeiffer, E.F.: Antigen-binding leucocytes in patients and in insulin-sensitized animals with delayed insulin allergy. Immunopathology Vth Int. Symposion p. 107. Ed. by P.A. Miescher und P. Graber, Basel/Stuttgart: Schwabe und Co. Publ. 1968.
Federlin, K.: Immunopathology of insulin. Monographs on Endocrinology6, 1–158 (1971).
Freytag, G., Klöppel, G.: Experimentelle Insulitis und Pankreatitis nach Immunseren gegen Pankreasextrakte verschiedener Reinheitsgrade. Beitr. path. Anat.39, 138 (1969).
Freytag, G., Klöppel, G., Howe, I.: Zur Pathogenese der experimentellen Insulitis. Verh. Dtsch. Ges. Path.53, 423 (1969).
Freytag, G., Menke, B.: Latenter Diabetes mellitus bei Meerschweinchen während der aktiven Immunisierung gegen Fremdinsulin. 16. Symp. Dtsch. Ges. Endokrinologie, p. 65. Berlin-Heidelberg-New York: 1970.
Freytag, G.: Immunpathologie des Diabetes mellitus. Veröffentlichungen aus der Morphologischen Pathologie. Vol. 88, 1–101 edit, by W. Giese, W. Büngler, G. Seifert und G. Peters. Stuttgart: Gustav Fischer Verlag 1972.
Freytag, G., Jansen, F.K., Klöppel, G.: Immune Reactions to Fractions of Crystalline Insulin I. Significance of Lymphoeytic Infiltrates in the Endocrine and Exocrine Pancreas of Mice. Diabetologia9, 185–188 (1973).
Jansen, F.K.: Tolerance to high and low doses of natural crystalline insulin. Diabetologia7, 290–292 (1971a).
Jansen, F. K.: The ability of MC-insulin or 1 X crystallized insulin in the development of immunological tolerance in mice. Diabetologia7, 485 (1971b) Abstr.
Klöppel, G., Altenähr, E., Freytag, G.: Studies on ultrastructure and immunology of the insulitis in rabbits immunized with insulin. Virchows Arch. Abt. A. Path. Anat.356, 1–15 (1972).
LeCompte, P.M., Legg, M.A.: Insulitis (lymphocytic infiltration of pancreatic islets) in late-onset diabetes. Diabetes21, 762–769 (1972).
Lienert, G.A.: Verteilungsfreie Methoden in der Biostatistik. Maisenheim am Glan: Verlag Anton Hain, 1962.
Meade, R.C., Klitgaard, H.M.: A simplified method for immuno-assay of human serum insulin. J. nucl. Med.3, 407–416 (1962).
Mirsky, I.R., Kawamura, N.: Heterogenicity of crystalline insulin. Endocrinology78, 1115–1119 (1966).
Renold, A.E., Soeldner, J.S., Steinke, J.: Immunologioal studies with homologous and heterologous pancreatic insulin in the cow. Ciba Foundation Colloquia Endocrinology15, 122 (1964).
Schichtkrull, J., Brange, J., Ege, H., Hallund, O., Heding, L.G., Christiansen, A.H., Jorgensen, K., Markussen, J., Stahnke, P., Sundby, F., Volund, A.: Proinsulin and related proteins. European Association for the Study of Diabetes. 5th annual meeting, Montpellier 1969.
Toreson, W.E., Feldman, R., Lee, J.C., Grodsky, G.M.: Pathology of diabetes mellitus produced in rabbits by means of immunization with beef insulin. Amer. J. clin. Path.42, 531 (1964).
Weigle, W.O.: Recent observations and concepts in immunological unresponsiveness and autoimmunity. Clin. exp. Immunol.9, 437–447 (1971).
Author information
Authors and Affiliations
Additional information
Published in part at the Congress of the European Association for the Study of Diabetes, Madrid 1972.
Supported by Landesamt für Forschung des Landes Nordrhein Westfalen, W. Germany.
1 True Sanger insulin=insulin cirresponding exactly to Sanger's formula.
Rights and permissions
About this article
Cite this article
Jansen, F.K., Freytag, G. Immune reactions to fractions of crystalline insulin. Diabetologia 9, 191–196 (1973). https://doi.org/10.1007/BF01219782
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01219782