Summary
Thyroxine treatment did not significantly affect the immediate insulin secretory response of the perfused rat pancreas, but it inhibited the late phase of D-glucose-induced insulin secretion. Thyroxine treatment did not inhibit D-glyceraldehyde-, D-mannose-, and tolbutamide-induced insulin release from the perfused pancreas. An increase in the D-glucose concentration of the perfusion medium as well as feeding of the rats did not restore insulin secretion after thyroxine treatment. The inhibition of D-glucose-induced insulin release in response to thyroxine treatment was reversed after addition of either D-glyceraldehyde, dihydroxyacetone, DL-glyceric acid, pyruvate, or α-ketobutyrate to the perfusion medium. Tolbutamide, L-glucose, D-fructose, D-mannose, L-lactate, and propionic acid were not able to overcome the inhibition of D-glucose-induced insulin secretion. Except for α-ketobutyrate all substances which were effective in reversing the inhibition of D-glucose-induced insulin release were glycolytic intermediates. Comparing the glycolytic α-ketoacid pyruvate and the non-glycolytic ketoacid α-ketobutyrate, the only part common to both substances was the ketoacid moiety. It is concluded from these findings that the ketoacid moiety of the α-ketoacids plays an important role in reversing the effect of thyroxine on D-glucose-induced insulin release.
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Lenzen, S., Joost, H.G. & Hasselblatt, A. The inhibition of insulin secretion from the perfused rat pancreas after thyroxine treatment. Diabetologia 12, 495–500 (1976). https://doi.org/10.1007/BF01219514
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DOI: https://doi.org/10.1007/BF01219514