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Synthesis of millimole amounts of14C- and2D-labelled primidone, phenylethylmalonamide and phenobarbital for high-sensitivity detection in biological materials

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Summary

Syntheses of millimole quantities of14C- and2D-labelled primidone, phenylethylmalonamide and phenobarbital are described. The labels are incorporated in positions that permit quantitative recovery in the metabolites. Methods are given for isolating the compounds by countercurrent distribution and thin-layer chromatography. Mass spectra of2D-labelled and non-labelled primidone are presented. Detection, identification and quantitation of the compounds and their metabolites in biological material by radiotracer and GC-MS procedures are discussed.

Zusammenfassung

Synthesen von Millimolen14C- und2D-markierten Primidons, Phenyläthylmalonamids und Phenobarbitals wurden beschrieben. Die Markierung erfolgte an Stellen, die eine quantitative Auffindung in den betreffenden Metaboliten ermöglichen.

Methoden zur Isolierung der genannten Verbindung durch Gegenstromverteilung und Dünnschichtchromatographie wurden angegeben Massenspektren von2D-markiertem und nicht markiertem Primidon wurden angegeben. Nachweis, Identifizierung und Bestimmung der Verbindungen und ihrer Stoffwechselprodukte in biologischem Material mit Radiotracern bzw. durch Gaschromatographie und Massenspektrometrie wurden erörtert.

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References

  1. J. M. Fujimoto, W. H. Mason, and M. Murphy, J. Pharmacol. Exp. Therap.159, 379 (1968).

    Google Scholar 

  2. G. L. Plaa, J. M. Fujimoto, and C. H. Hine, J. Amer. Med. Assoc.168, 1769 (1958).

    Google Scholar 

  3. M. T. Bush, Analyt. Biochem.32, 145 (1969).

    Google Scholar 

  4. M. T. Bush and P. M. Densen, Analyt. Chemistry20, 121 (1948).

    Google Scholar 

  5. M. T. Bush, Microchem. J.5, 73 (1961).

    Google Scholar 

  6. W. Hackbart and M. Hartman, J. prakt. Chem.14, 1 (1961).

    Google Scholar 

  7. D. L. Tabern and E. H. Volweiler, J. Amer. Chem. Soc.57, 1961 (1935).

    Google Scholar 

  8. B. Chechelska, B. Morawski, and A. Zuponski, Polish Patent44, 676 (1963).

    Google Scholar 

  9. L. F. Fieser and M. Fieser, Reagents for Organic Syntheses, New York: Wiley. 1967. p. 1276.

    Google Scholar 

  10. M. T. Bush, H. J. Sekerke, M. Vore, B. J. Sweetman, and J. T. Watson, in P. D. Klein and L. J. Roth, eds., Proceedings of Seminar on the Use of Stable Isotopes, Springfield, Va., p. 233, August, 1973.

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Authors and Affiliations

  1. Department of Pharmacology, Vanderbilt University, 37203, Nashville, Tenn., USA

    John D. Alvin & Milton T. Bush

Authors
  1. John D. Alvin
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  2. Milton T. Bush
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Alvin, J.D., Bush, M.T. Synthesis of millimole amounts of14C- and2D-labelled primidone, phenylethylmalonamide and phenobarbital for high-sensitivity detection in biological materials. Mikrochim Acta 63, 685–696 (1975). https://doi.org/10.1007/BF01217330

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  • DOI: https://doi.org/10.1007/BF01217330

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