Summary
Aldehyde-fuchsin-positive B granules were found in rabbits ranging from 24-day embryos to adult animals. Ultrastructurally, the B granules were of two types:dark andpale; they displayed different fixation properties. The granules of foetal and neonatal rabbits were almost exclusively of the pale type and were distributed mainly along the cell border. B cells from older rabbits showed a progressive increase in the number of granules and in the proportion of dark to pale granules; they also showed changes in the granule distribution pattern. Despite morphological differences, the pancreases from 24-day embryos, 30-day embryos, and adult rabbits all showed a significant insulin release in response to glucose challengein vitro. Non-necrotic B cells from pancreas after glucose challenge presented no obvious changes in aldehyde-fuchsin positivity, granule distribution, proportion of dark to pale granules, or the extent of emiocytosis.
Résumé
Les granules B réagissant positivement à l'aldéhyde-fuchsine, peuvent être mis en évidence dans le pancréas de lapin à partir du vingt-quatrième jour de la vie embryonnaire jusqu'à l'âge adulte. On peut identifier en microscopie électronique deux types de granules B avec des propriétés de fixation différente: granules denses et granules clairs. Dans le foetus ou le nouveau-né de lapin les granules sont presque exclusivement du type clair et situés principalement à la périphérie de la cellule. Plus tard, à un âge plus avancé, on constate une augmentation progressive des granules denses par rapport aux granules clairs, ainsi qu'un accroissement du degré de granulation et un changement dans la distribution cytoplasmique des granules. Malgré les différences morphologiques, la libération d'insuline par le pancréas foetal et celui de l'adulte, incubés dans les conditionsin vitro, a été augmentée d'une façon significative en présence de concentration élevée de glucose. Cependant, l'étude morphologique des cellules B du pancréas, après l'incubation, n'a pas pu révéler des changements évidents dans la positivité à l'aldéhyde-fuchsine, la distribution ou la proportion des deux types de granules, ou dans le degré de l'émiocytose.
Zusammenfassung
Vom 24 Tage alten Kaninchenembryo bis zum erwachsenen Tier wurden aldehydfuchsinpositive B-Granula gefunden. Elektronenmikroskopisch unterscheiden wir zwei Typen von Granula, helle und dunkle, die verschiedene Fixationseigenheiten darstellen. Die Granula von foetalen und neugeborenen Kaninchen waren fast ausschließlich vom hellen Typ und vornehmlich entlang dem Zellrand verteilt. B-Zellen von älteren Kaninchen zeigen die fortschreitende Erhöhung der Proportion dunkler zu heller Granula, sowie eine allmähliche Zunahme und Veränderung in der Anordnung der Granula. Trotz morphologischer Unterschiede reagieren Pankreata von 24 Tage alten Embryos, 30 Tage alten Embryos und von erwachsenen Kaninchen mit einer deutlichen Insulinfreisetzung bei Glucosestimulationin vitro. Jedoch ergaben Untersuchungen von nicht nekrotischen B-Zellen beim inkubierten Pankreas keine deutliche Änderung in der Aldehydefuchsinpositivität, Granulaverteilung, im Verhältnis von dunklen zu hellen Granula und dem Ausmaß der Emiocytosis.
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Beitcosme, S.A., Wilson, M.B., Aleyassine, H. et al. Rabbit pancreatic B cell. Morphological and functional studies during embryonal and post-natal development. Diabetologia 6, 399–411 (1970). https://doi.org/10.1007/BF01212072
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DOI: https://doi.org/10.1007/BF01212072